Immunolocalization of interleukin-4 in eosinophils in the bronchial mucosa of atopic asthmatics
| Autor: | T. H. Van Der Kwast, Henk C. Hoogsteden, T. Thepen, G. M. Moller, T. A. W. De Jong, S. E. Overbeek, Annet F Wierenga-Wolf |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Pulmonary and Respiratory Medicine
Pathology medicine.medical_specialty Biopsy Clinical Biochemistry Bronchi Cytoplasmic Granules Reference Values medicine Humans Microscopy Immunoelectron Molecular Biology Interleukin 4 Eosinophil cationic protein Mucous Membrane biology Chemistry Bronchial mucosa Interleukin Cell Biology Allergens respiratory system Eosinophil Immunohistochemistry Primary and secondary antibodies Asthma Eosinophils medicine.anatomical_structure Immunology Ultrastructure biology.protein Interleukin-4 |
| Zdroj: | American Journal of Respiratory Cell and Molecular Biology. 14:439-443 |
| ISSN: | 1535-4989 1044-1549 |
| DOI: | 10.1165/ajrcmb.14.5.8624248 |
| Popis: | Several studies have shown that human eosinophils can synthesize and release a number of cytokines. The aim of this study was to investigate whether eosinophils contain interleukin (IL)-4 protein. We examined the ultrastructural localization of IL-4 in eosinophils in bronchial mucosal biopsies of 29 nonsmoking atopic asthmatics and 7 controls. No eosinophils were detected in the bronchial mucosa of controls. In the eosinophils (n = 42) of the asthmatics, IL-4 was localized to the electron-dense crystalloid core compartment of 85% of the secondary or specific eosinophil granules (n = 468). Other structures in the eosinophils were unlabeled. Control sections, incubated with an irrelevant primary antibody, were negative. This study demonstrates that pre-formed IL-4 is stored in the secondary eosinophil granules. These results were extended by light microscopic immunodouble-staining for IL-4 protein and eosinophil cationic protein, which showed that a subpopulation of activated eosinophils express IL-4 immunoreactivity in bronchial mucosal biopsies of asthmatics as well as controls. These data indicate that eosinophils may be an important source of IL-4 in allergic inflammation. |
| Databáze: | OpenAIRE |
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