Isolation and characterization of inositol sphingophospholipids from Phytophthora parasitica Dastur
| Autor: | V. Pivot, Florence Fournol, Maud Bruneteau, Corinne Gandon, Michel Becchi |
|---|---|
| Přispěvatelé: | Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 1997 |
| Předmět: |
Phytophthora
Fungus Carnation Spectrometry Mass Fast Atom Bombardment Biology Biochemistry 03 medical and health sciences chemistry.chemical_compound [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Inositol Inositol phosphate Chromatography High Pressure Liquid Mycelium 030304 developmental biology chemistry.chemical_classification Sphingolipids 0303 health sciences Molecular mass fungi 030302 biochemistry & molecular biology Organic Chemistry Cell Biology Phytophthora parasitica biology.organism_classification Molecular Weight chemistry Chromatography Thin Layer |
| Zdroj: | Lipids Lipids, Springer Verlag, 1997, 32, pp.359-362. ⟨10.1007/s11745-997-0045-9⟩ |
| ISSN: | 1558-9307 0024-4201 |
| DOI: | 10.1007/s11745-997-0045-9 |
| Popis: | International audience; Several inositol sphingophospholipids (ISPL) were isolated from mycelia of Phytophthora parasitica Dastur, a phytopathogenic fungus of carnation. The ISPL structures were determined by fast atom bombardment. All ISPL consisted of ceramides linked to inositol phosphate. We investigated the effect of growth conditions on the ISPL produced in four different media that are commonly used for fungal cultures. We showed that P. parasitica Dastur synthesized four major classes of compounds with molecular weights of M(r) = 751, 807, 835, and 849 containing the 16:1 base and the 16:0 or 20:0 or 22:0 or 22h:1 N-acyl group. The relative abundance of the different ISPL is dependent on growth conditions.Several inositol sphingophospholipids (ISPL) were isolated from mycelia of Phytophthora parasitica Dastur, a phytopathogenic fungus of carnation. The ISPL structures were determined by fast atom bombardment. All ISPL consisted of ceramides linked to inositol phosphate. We investigated the effect of growth conditions on the ISPL produced in four different media that are commonly used for fungal cultures. We showed that P. parasitica Dastur synthesized four major classes of compounds with molecular weights of M(r) = 751, 807, 835, and 849 containing the 16:1 base and the 16:0 or 20:0 or 22:0 or 22h:1 N-acyl group. The relative abundance of the different ISPL is dependent on growth conditions. |
| Databáze: | OpenAIRE |
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