Imidacloprid affects rat liver mitochondrial bioenergetics by inhibiting FoF1-ATP synthase activity
| Autor: | Fábio Erminio Mingatto, Anilda Rufino de Jesus Santos Guimarães, Marcos A. Maioli, Paulo Francisco Veiga Bizerra |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Oligomycin Bioenergetics biology ATP synthase Health Toxicology and Mutagenesis Adenine nucleotide translocator Respiratory chain 010501 environmental sciences Mitochondrion Pharmacology Toxicology 01 natural sciences ANT 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Imidacloprid biology.protein 0105 earth and related environmental sciences |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1087-2620 1528-7394 |
| DOI: | 10.1080/15287394.2018.1437581 |
| Popis: | Made available in DSpace on 2018-12-11T17:35:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-04-18 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Imidacloprid (IMD) is a neonicotinoid insecticide widely used in crops, pets, and on farm animals for pest control. Several studies were conducted examining the adverse effects of IMD on animals often exhibiting hepatic damage. The aim of this study was to determine the effects of IMD on bioenergetics of mitochondria isolated from rat liver. Imidacloprid (50–200 µM) produced a concentration-dependent decrease in oxygen consumption and ATP production without markedly affecting mitochondrial membrane potential (MMP). Oxygen consumption experiments showed that IMD did not significantly affect the respiratory chain, and this was similar to findings with oligomycin and carboxyatractyloside, suggesting a direct action on FoF1-ATP synthase and/or the adenine nucleotide translocator (ANT). Imidacloprid inhibited FoF1-ATP synthase activity only in disrupted mitochondria and induced a partial inhibition of ADP-stimulated depolarization of the MMP. Our results indicate that IMD interacts specifically with FoF1-ATP synthase resulting in functional inhibition of the enzyme with consequent impairment of mitochondrial bioenergetics. These effects of IMD on mitochondrial bioenergetics may be related to adverse effects of this insecticide on the liver. College of Agricultural and Technological Sciences São Paulo State University (Unesp) School of Veterinary Medicine São Paulo State University (Unesp) College of Agricultural and Technological Sciences São Paulo State University (Unesp) School of Veterinary Medicine São Paulo State University (Unesp) FAPESP: 2015/19549-8 |
| Databáze: | OpenAIRE |
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