Exoelectrogenic capacity of host microbiota predicts lymphocyte recruitment to the gut
| Autor: | Craig L. Franklin, Aaron C. Ericsson, Catherine E. Hagan, Daniel J. Davis |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Physiology
Microorganism Segmented filamentous bacteria Molecular Sequence Data Enzyme-Linked Immunosorbent Assay Gut flora Real-Time Polymerase Chain Reaction Electrolysis Statistics Nonparametric Microbiology Exoelectrogen Feces Mice Immune system Microscopy Electron Transmission Call for Papers: Gut Microbiota in Health and Disease Cell Movement Proteobacteria Genetics Animals Fluorometry Lymphocytes Gene Library Microscopy Confocal Base Sequence biology Reverse Transcriptase Polymerase Chain Reaction Computational Biology Sequence Analysis DNA Bacteria Present biology.organism_classification Adoptive Transfer Immunohistochemistry Electrophysiological Phenomena Gastrointestinal Microbiome Gastrointestinal Tract Mice Inbred C57BL Gene Expression Regulation Gastric Mucosa Microscopy Electron Scanning Ex vivo |
| Zdroj: | Physiological Genomics. 47:243-252 |
| ISSN: | 1531-2267 1094-8341 |
| Popis: | Electrotaxis, directional cell movement in response to an electric potential, has been demonstrated in a wide range of cell types including lymphocytes. Exoelectrogens, microorganisms capable of generating electrical currents, have been identified in microbial fuel cells. However, no studies have investigated exoelectrogenic microbes in fresh feces or the effects of an exoelectrogenic microbiota on the host organism. Here we show that commensal gut microbial populations differ in their capacity for electrical current production by exoelectrogens and that those differences are predictive of increased lymphocyte trafficking to the gut in vivo, despite the lack of increased production of canonical lymphocyte-specific chemokines. Additionally, we demonstrate that the difference in current production between mice purchased from different commercial sources correlates reproducibly with the presence or absence of segmented filamentous bacteria, and while our data do not support a direct role for segmented filamentous bacteria in ex vivo current production, an exoelectrogenic microbiota can be transferred in vivo via mucosa-associated bacteria present in the ileum. Moreover, we detect upregulation of microbial genes associated with extracellular electron transfer in feces of mice colonized with exoelectrogenic microbiota containing segmented filamentous bacteria. While still correlative, these results suggest a novel means by which the gut microbiota modulates the recruitment of cells of the immune system to the gut. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|