Simultaneous increase in labeling of nuclear RNA and of DNA associated acidic nuclear protein
| Autor: | L. Fanshier, L.S. Hnilica, T.T. Crocker, V. Holoubek |
|---|---|
| Rok vydání: | 1966 |
| Předmět: |
Arginine
Cell Biology In Vitro Techniques General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Protein A/G medicine Animals RNA Neoplasm General Pharmacology Toxicology and Pharmaceutics Nuclear protein Amino Acids Carcinoma Ehrlich Tumor Uridine Cell Nucleus Carbon Isotopes Nuclear cap-binding protein complex RNA General Medicine DNA Neoplasm Molecular biology Neoplasm Proteins medicine.anatomical_structure Nucleoproteins chemistry Biochemistry biology.protein Specific activity DNA |
| Zdroj: | Life sciences. 5(18) |
| ISSN: | 0024-3205 |
| Popis: | After removal of acid soluble nuclear protein and RNA from Ehrlich ascites cell nuclei the DNA was extracted with hot 0.5 N perchloric acid together with a protein, or proteins, representing from 12 to 16 percent of the total weight of the DNA. The amino acid composition of this protein has a prepronderance of acidic over basic amino acids. After 30 minutes labeling with 14C arginine of cells maintained in minimal medium, the specific activity of this protein, or proteins, was significantly higher than the specific activity of acid soluble or residual nuclear proteins. The labeling of this protein, or proteins, is proportional to the labeling of nuclear RNA with 3H uridine. It is proposed that this protein, or proteins, is involved in the control of RNA synthesis on the DNA template. |
| Databáze: | OpenAIRE |
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