Anti-sickling nature of dimethyl adipimidate
| Autor: | Kotaro Yamaoka, G. Larry Cottam, Michael R. Waterman, A. Henry Chuang |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Hemoglobin s
Erythrocytes Magnetic Resonance Spectroscopy Chemistry Adipates Electrophoresis Starch Gel Biophysics Cell Biology Anemia Sickle Cell Imides Biochemistry In vitro chemistry.chemical_compound Starch gel electrophoresis Hemoglobins Dimethyl Adipimidate Oxyhemoglobins Water proton Humans Hemoglobin Bifunctional Molecular Biology |
| Zdroj: | Biochemical and biophysical research communications. 63(3) |
| ISSN: | 0006-291X |
| Popis: | Dimethyl adipimidate (DMA), a bifunctional cross-linking agent, is known to be a powerful anti-sickling agent in vitro . The hemoglobin isolated from DMA-treated erythrocytes is heterogeneous with respect to charge and size as shown by starch gel electrophoresis and sedimentation velocity studies; some hemoglobin tetramers being cross-linked together resulting in high molecular weight species. Measurement of transverse water proton relaxation times of DMA-treated sickle erythrocytes shows that while some aggregation may take place within deoxygenated erythrocytes, it is not of sufficient extent to cause the cells to change shape. Therefore, it is suggested that the anti-sickling effect of DMA is due to its ability to react with hemoglobin S inside sickle erythrocytes and inhibit the aggregation process. |
| Databáze: | OpenAIRE |
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