Immunoglobulin G1 subclass responses can be used to detect specific allergy to the house dust mites Dermatophagoides farinae and Dermatophagoides pteronyssinus in atopic dogs
| Autor: | Chanettee Chanthick, Nathrada Khantavee, A. Tungtrongchitr, Nitat Sookrung, Sanipa Suradhat, Sittiruk Roytrakul, Nuvee Prapasarakul, Navapon Techakriengkrai |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Allergy 040301 veterinary sciences Dermatophagoides pteronyssinus Enzyme-Linked Immunosorbent Assay Immunoglobulin E medicine.disease_cause Subclass Serology Dermatitis Atopic 0403 veterinary science 03 medical and health sciences Allergen Dogs medicine Animals Dog Diseases Antibody 030304 developmental biology Skin Tests 0303 health sciences lcsh:Veterinary medicine General Veterinary biology Dermatophagoides farinae House dust mites business.industry Canine atopic dermatitis 04 agricultural and veterinary sciences General Medicine Atopic dermatitis Biomarker Allergens medicine.disease Immunoglobulin G Immunology biology.protein Immunoglobulin G1 subclass Biomarker (medicine) lcsh:SF600-1100 Female business Research Article |
| Zdroj: | BMC Veterinary Research, Vol 17, Iss 1, Pp 1-10 (2021) BMC Veterinary Research |
| ISSN: | 1746-6148 |
| Popis: | Background In dogs with atopic dermatitis, intradermal testing (IDT) or allergen specific IgE serological testing are routinely employed to identify causative allergens. These allergens can then be used for allergen-specific immunotherapy and allergy management. The clinical relevance of this testing is affected by the source of allergen, and other biomarkers that are more related to specific allergens still need to be identified. The aim of this study was to investigate levels of specific IgE, total IgG, and IgG1 and IgG2 subclasses against the local house dust mites (HDM) Dermatophagoides farinae (DF) and D. pteronyssinus (DP) as biomarkers by using in-house ELISAs in healthy (n = 33) and atopic dogs (AD) (n = 44) that were either positive or negative by IDT to HDM. Results Being over 3 years of age was a risk factor for AD (Odds Ratio (OD) = 4.10, 95% Confidence interval (CI) 1.57–10.75, p = 0.0049), but there was no relation to IDT outcomes (OR = 0.9091, 95% CI 0.22–3.74, p = 1.00). High levels of all antibody isotypes (IgE, IgG, IgG1 and IgG2) against HDM were found in aged healthy dogs (> 3 years old). In AD, HDM-IgE and IgG1 levels were higher in dogs that were IDT positive to HDM than in IDT negative animals. Levels of IgE and IgG1 could be used to distinguish the specific allergens, whereas total IgG and IgG2 levels were not different between IDT-positive and IDT-negative AD. By the receiver operating characteristic curve at a false-positive rate = 0.10, both IgE and IgG1 showed better sensitivity than IgG and IgG2. Similar to IgE, serum IgG1 concentration was also relevant to IDT outcomes. Conclusions Our in-house ELISAs coated with local HDM were useful for evaluating antibody levels, and we propose use of the HDM-specific IgG1 subclass as a biomarker to detect HDM specific allergens in AD, potentially together with an IgE based platform. |
| Databáze: | OpenAIRE |
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