Virus purification by CsCl density gradient using general centrifugation
| Autor: | Tadahiro Nasukawa, Masahiro Sakaguchi, Takako Ujihara, Hironobu Murakami, Sumire Ota, Keijirou Mizukami, Shigenobu Matsuzaki, Satoshi Taharaguchi, Jumpei Uchiyama |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Strain (chemistry) Density gradient viruses 030106 microbiology Cesium General Medicine Biology Virology Virus 03 medical and health sciences 030104 developmental biology Chlorides Centrifugation Density Gradient Bacteriophages Centrifugation Density gradient ultracentrifugation Ultracentrifuge |
| Zdroj: | Archives of Virology. 162:3523-3528 |
| ISSN: | 1432-8798 0304-8608 |
| Popis: | Virus purification by cesium chloride (CsCl) density gradient, which generally requires an expensive ultracentrifuge, is an essential technique in virology. Here, we optimized virus purification by CsCl density gradient using general centrifugation (40,000 × g, 2 h, 4 °C), which showed almost the same purification ability as conventional CsCl density gradient ultracentrifugation (100,000 × g, 1 h, 4 °C) using phages S13' and φEF24C. Moreover, adenovirus strain JM1/1 was also successfully purified by this method. We suggest that general centrifugation can become a less costly alternative to ultracentrifugation for virus purification by CsCl densiy gradient and will thus encourage research in virology. |
| Databáze: | OpenAIRE |
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