Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection
| Autor: | S Kurz, K Manske, Annika Schneider, Thomas Misgeld, Percy A. Knolle, Sebastian Felix Weissmann, Michaela Aichler, Mathias Heikenwalder, Hans Zischka, Dirk Wohlleber, Marianne K. Janas |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
metabolism [Mitochondria Liver] ultrastructure [Mitochondria Liver] viruses Science Mitochondria Liver Mitochondrion Biology Mitochondrial Size Article law.invention Flow cytometry 03 medical and health sciences 0302 clinical medicine law ultrastructure [Hepatocytes] Organelle medicine Animals Humans virology [Hepatocytes] Membrane Potential Mitochondrial metabolism [Virus Diseases] Multidisciplinary medicine.diagnostic_test HEK 293 cells Phenotype ddc Cell biology Mice Inbred C57BL Crosstalk (biology) 030104 developmental biology HEK293 Cells Virus Diseases Viral infection Organelle Size Recombinant DNA Hepatocytes Medicine ddc:600 virology [Mitochondria Liver] 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019) Sci. Rep. 9:8492 (2019) Scientific Reports Scientific reports 9(1), 8492 (2019). doi:10.1038/s41598-019-44922-9 |
| ISSN: | 2045-2322 |
| Popis: | Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry. Resolution at the level of tagged individual mitochondria revealed changes in mitochondrial size, membrane potential and displayed a fragile phenotype during viral infection of cells. Thus, single-organelle and multi-parameter resolution allows to explore altered energy metabolism and antiviral defence by tagged mitochondria selectively in virus-infected cells and will be instrumental to identify viral immune escape and to develop and monitor novel mitochondrial-targeted therapies. |
| Databáze: | OpenAIRE |
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