Bioassay-Guided Isolation of an Anti-Ulcer Compound, Tagitinin C, from Tithonia diversifolia: Role of Nitric Oxide, Prostaglandins and Sulfhydryls
| Autor: | Juan Rodríguez-Silverio, Jesús Arrieta, Adelfo Reyes-Ramírez, María Elena Sánchez-Mendoza, Leticia Cruz Antonio, Luis Martínez Jiménez |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Male
Tithonia diversifolia Asteraceae tagitinin C gastroprotection medicinal plants Pharmaceutical Science Nitric Oxide Article Analytical Chemistry Nitric oxide lcsh:QD241-441 chemistry.chemical_compound lcsh:Organic chemistry Drug Discovery Animals Bioassay Sulfhydryl Compounds Rats Wistar Physical and Theoretical Chemistry Medicinal plants Dichloromethane Dose-Response Relationship Drug biology Traditional medicine Chemistry Experimental model Organic Chemistry Tithonia Anti-Ulcer Agents biology.organism_classification Rats Chemistry (miscellaneous) Prostaglandins Arginine methyl ester Molecular Medicine Biological Assay Sesquiterpenes Tagitinin C |
| Zdroj: | Molecules; Volume 16; Issue 1; Pages: 665-674 Molecules, Vol 16, Iss 1, Pp 665-674 (2011) Molecules |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules16010665 |
| Popis: | Tithonia diversifolia is a medicinal plant from the Municipality of Suchiapa, Chiapas, Mexico, that according to local folk medicine is considered useful in the treatment of gastric ulcers. The aim of the present study was to investigate the gastroprotective activity of T. diversifolia by using an ethanol-induced gastric ulcer experimental model in male Wistar rats. The results showed that T. diversifolia had gastroprotective activity, and that the dichloromethane extract had the highest protective activity (close to 90% when using doses between 10 to 100 mg/kg), and that further the compound tagitinin C isolated from this extract was the main active gastroprotective agent. Rats treated with tagitinin C suspended in Tween 80 at 1, 3, 10 and 30 mg/kg showed 37.7, 70.1, 100, and 100% gastroprotection, respectively. The effect elicited by tagitinin C (30 mg/kg) was not attenuated by pretreatment with either N(G)-nitro-L-arginine methyl ester (70 mg/kg, i.p.), a nitric oxide (NO) synthase inhibitor, N-ethylmaleimide (10 mg/kg, s.c.), a blocker of sulfhydryl groups, or indomethacin (10 mg/kg, s.c.), a blocker of prostaglandin synthesis, which suggests that the gastroprotective mechanism of action of this sesquiterpene lactone does not involve NO, sulfhydryl groups or prostaglandins. |
| Databáze: | OpenAIRE |
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