Probiotic supplementation reduces the risk of bacterial translocation in experimental short bowel syndrome
| Autor: | Pablo Aldazabal, Asensio Ab, C. Vidales, P. Zubillaga, García-Arenzana Jm, I. Zubillaga, Eizaguirre I, N. García Urkia |
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| Rok vydání: | 2002 |
| Předmět: |
Short Bowel Syndrome
medicine.medical_specialty medicine.medical_treatment Gastroenterology Inflammatory bowel disease law.invention Cecum Probiotic law Internal medicine Medicine Mesenteric lymph nodes Animals Rats Wistar Bifidobacterium Gastrointestinal tract biology Gram-Negative Aerobic Bacteria business.industry Probiotics General Medicine Bowel resection Short bowel syndrome medicine.disease biology.organism_classification Rats medicine.anatomical_structure Bacterial Translocation Pediatrics Perinatology and Child Health Immunology Surgery business |
| Zdroj: | Journal of pediatric surgery. 37(5) |
| ISSN: | 1531-5037 |
| Popis: | Probiotics are live organisms that survive passage through the gastrointestinal tract and have beneficial effects on the host. Lactobacillus and Bifidobacterium have been recommended for cholesterol lowering, acute diarrhea, prevention of cancer, or inflammatory bowel disease. On the other hand, after massive bowel resection, bacterial overgrowth is frequent and favors bacterial translocation (BT). The possible beneficial effects of Bifidobacterium lactis (BL) administration on BT in experimental short bowel syndrome (SBS), have not been investigated. The aim of this study was to test the hypothesis that BL administration decreases BT in SBS in animals fed orally.One hundred twenty-eight adult Wistar rats fed orally with standard rat chow and tap water "ad libitum" were maintained in individual metabolic cages for 10 days and divided into 3 groups: control group (n = 71): nonmanipulated animals; RES group (n = 39): 80% gut resection from 10 cm beyond the angle of Treitz to 10 cm above the cecum; RES-PRO group (n = 18): same resection and daily 7.8 x 10(8) CFU B Lactis administration, after orogastric intubation. At the end of the experiment they were killed, and mesenteric lymph nodes (MLN) and peripheral and portal blood specimens were recovered and cultured. Bacterial identification in blood was made by conventional methods, and MLN culture was considered positive with a growth over 100 CFU/g.Bacterial translocation was detected in 6% of control group rats. The incidence of BT in the RES group was 87% (34 of 39), whereas only 50% (9 of 18) of RES-PRO animals had BT (P.05). The relative risk reduction (RRR) was 0.43 (95% Cl 0.14 to 0.72), and the number needed to treat (NNT) was 3 (95% Cl 2 to 8). In other words, animals that received BL had the risk of BT reduced by 43% (RRR of 0.43), and of every 3 animals treated, 1 is expected to be free of BT (NNT of 3).Administration of B Lactis reduces the incidence of BT in adult Wistar rats after 80% gut resection. |
| Databáze: | OpenAIRE |
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