Transfection of Cultured Eukaryotic Cells Using Cationic Lipid Reagents
| Autor: | Valentina C. Ciccarone, Pamela Hawley-Nelson, Meredith L. Moore |
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| Rok vydání: | 1999 |
| Předmět: |
Insecta
Genetic enhancement Transgene Transfection Jurkat Cells Mice Cations Gene expression Cell Adhesion Protein biosynthesis Animals Humans Luciferase RNA Small Interfering Molecular Biology Cells Cultured Cell growth Chemistry fungi RNA DNA General Medicine Lipids Cell biology Eukaryotic Cells Biochemistry Cell culture NIH 3T3 Cells Indicators and Reagents RNA transfection Baculoviridae |
| Zdroj: | Current Protocols in Molecular Biology. 48 |
| ISSN: | 1934-3647 1934-3639 |
| DOI: | 10.1002/0471142727.mb0904s48 |
| Popis: | The development of high-efficiency methods for the introduction of functional genetic material into eukaryotic cells using cationic lipids has accelerated biological research in the studies of gene expression, control of cell growth, and cell lineage. Transfection mediated by cationic lipids is commonly used in industrial protein production as well as in some clinical gene therapy protocols. This unit describes how to perform transfection of adherent and suspension cells, insect cells, and RNA transfection using cationic lipid reagents. Curr. Protoc. Mol. Biol. 81:9.4.1-9.4.17. © 2008 by John Wiley & Sons, Inc. Keywords: transfection; luciferase; cationic lipid; cell culture; transgene; transient gene expression; stable gene expression |
| Databáze: | OpenAIRE |
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