Physiological assessment of muscle strength in vitro after direct injection of doxorubicin into rabbit sternocleidomastoid muscle

Autor: Paul A. Iaizzo, J. H. Falkenberg, Linda K. McLoon
Rok vydání: 2001
Předmět:
Zdroj: Movement Disorders. 16:683-692
ISSN: 1531-8257
0885-3185
DOI: 10.1002/mds.1125
Popis: Doxorubicin chemomyectomy is a potent method for the permanent removal of a muscle or group of muscles after direct local injection, and has been used successfully to treat blepharospasm and hemifacial spasm patients. The effi- cacy of doxorubicin chemomyectomy on reducing muscle strength after direct injection of doxorubicin into rabbit sterno- cleidomastoid muscle was tested. One- and 6-month postinjec- tion force assessment was performed in vitro to measure alter- ations in peak twitch and tetanic force generation, as well as fatigue responses for the treated muscles compared to control. There were significant reductions of both twitch and tetanic peak amplitudes in the doxorubicin-treated muscles. One month after treatment, the decreases in force were greater after 2 mg doxorubicin injections than after 1 mg doxorubicin. While there was a significant reduction in force generation after doxo- rubicin treatment, fatigue resistances for the doxorubicin- treated muscles were increased compared to the controls. There were significant reductions in muscle mass after doxorubicin treatment, and by 6 months, the myosin heavy chain isoform distribution was similar to normal sternocleidomastoid, except for an increase in slow myosin-positive fibers. Doxorubicin chemomyectomy resulted in a significant reduction in func- tional force generation in the treated sternocleidomastoid muscles. These findings suggest a potential clinical use of doxorubicin chemomyectomy to treat cervical dystonia pa- tients. © 2001 Movement Disorder Society.
Databáze: OpenAIRE
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