Antigenic characterization of endothelial cell-derived microparticles and their detection ex vivo
| Autor: | E. W. Meesters, M. N. Abid Hussein, Rienk Nieuwland, Auguste Sturk, F. P. H. Th. M. Romijn, N. Osmanovic |
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| Přispěvatelé: | ACS - Amsterdam Cardiovascular Sciences, CCA -Cancer Center Amsterdam, Laboratory Specialized Diagnostics & Research, Laboratory for General Clinical Chemistry |
| Rok vydání: | 2003 |
| Předmět: |
Adult
Umbilical Veins medicine.drug_class Population Monoclonal antibody Exocytosis Umbilical vein Flow cytometry E-selectin medicine Humans Lupus Erythematosus Systemic Antigens Particle Size education education.field_of_study biology medicine.diagnostic_test Endothelial Cells Hematology Middle Aged Flow Cytometry Molecular biology Endothelial stem cell Case-Control Studies biology.protein Female Endothelium Vascular Antibody E-Selectin Biomarkers Ex vivo Interleukin-1 |
| Zdroj: | Journal of thrombosis and haemostasis, 1(11), 2434-2443. Wiley-Blackwell |
| ISSN: | 1538-7836 1538-7933 |
| DOI: | 10.1046/j.1538-7836.2003.00455.x |
| Popis: | Background: Endothelial activation and dysfunction are associated with several diseases. However, hardly any specific markers are available. Microparticles (MP) from endothelial cells (EC; EMP) were reported in patient groups and healthy individuals. The antibodies used to detect EMP, however, were mainly directed against antigens without EC specificity. Objectives: We evaluated the antigens on EC and EMP to establish proper markers for EMP detection. Methods: EMP were isolated from supernatants of resting and interleukin (IL)-1alpha activated human umbilical vein EC (HUVEC; n = 3; 0-72 h), stained with annexin V and monoclonal antibodies, and analyzed by flow cytometry. Human platelet-MP (PMP), the main MP population in plasma, were prepared in vitro. EMP and PMP were studied in plasma from systemic lupus erythematosus (SLE) patients (n = 11) and healthy, individuals (n = 10). Results: Platelet-endothelial cell adhesion molecule-1 (PECAM-1), alpha(nu) and beta(3) were constitutively exposed on HUVEC, but (almost) absent on EMP ( 80%) and (subpopulations of) EMP exposed E-selectin and tissue factor. PMP strongly exposed PECAM-1, beta(3), and glycoprotein (GP)Ib (CD42b), but not a, or E-selectin. GPIb and P-selectin (CD62P) were absent on EMP. Plasma samples contained 0.5% MP staining for E-selectin and/or alpha(nu). Plasma from one SLE patient contained E-selectin exposing MP (21%), but little alpha(nu)-positive MR Conclusions: EC release EMP in vitro. The antigenic phenotype of EMP released from resting and IL-1alpha-stimulated EC differs among each other as well as from resting and stimulated EC, respectively. E-selectin exposed on IL-1alpha-stimulated EC is a valid marker for EMP detection ex vivo to establish endothelial cell activation |
| Databáze: | OpenAIRE |
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