Distinct mouse bone marrow macrophage precursors identified by differential expression of ER‐MP12 and ER‐MP20 antigens
| Autor: | Jane S. A. Voerman, Willem van Ewijk, Pieter J. M. Leenen, Johannes C. M. van der Loo, Marella F. T. R. de Bruijn, Walentina A. T. Slieker |
|---|---|
| Přispěvatelé: | Immunology, Hematology |
| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.drug_class Cellular differentiation Immunology Antigens Differentiation Myelomonocytic Macrophage-1 Antigen Bone Marrow Cells Biology Monoclonal antibody Immunophenotyping Mice Precursor cell medicine Animals Immunology and Allergy Macrophage Macrophage Colony-Stimulating Factor Macrophages Cell Differentiation Mononuclear phagocyte system Cell sorting Molecular biology Mice Inbred C57BL medicine.anatomical_structure Female Bone marrow |
| Zdroj: | European Journal of Immunology, 24(10), 2279-2284. Wiley-VCH |
| ISSN: | 0014-2980 |
| Popis: | The characterization of early branch points in the differentiation of leukocytes requires identification of precursor cells in the bone marrow. Recently, we produced two monoclonal antibodies, ER‐MP12 and ER‐MP20, which in two‐color flow‐cytometric analysis divide the murine bone marrow into six defined subsets. Here we show, using fluorescence‐activated cell sorting followed by macrophage colony‐stimulating factor‐stimulated culture in soft agar, that precursors of the mononuclear phagocyte system reside only within the ER‐MP12hi20−, ER‐MP12+20+ and ER‐MP12−20hi bone marrow subsets. Together, these subsets comprise 15% of nucleated bone marrow cells. Furthermore, we provide evidence that the macrophage precursors present in these subsets represent successive stages in a maturation sequence where the most immature ER‐MP12hi20− cells develop via the ER‐MP12+20+ stage into ER‐MP12−20hi monocytes. |
| Databáze: | OpenAIRE |
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