Thymocyte depletion affects neurotrophin receptor expression in thymic stromal cells
| Autor: | José A. Vega, Antonino Germanà, Pablo Perez-Pinera, Emilia Ciriaco, M. E. Del Valle, J. G. Prieto, O. García-Suárez |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Time Factors Receptor expression Apoptosis Tropomyosin receptor kinase B Tropomyosin receptor kinase A Receptor Nerve Growth Factor Dexamethasone apoptosis corticoids p75 Low-affinity nerve growth factor receptor Lymphocytes Receptor Caspase 3 Immunohistochemistry Cell biology Thymocyte Caspases Anatomy Neurotrophin medicine.medical_specialty Histology Stromal cell Down-Regulation Receptors Nerve Growth Factor Thymus Gland Biology Lymphocyte Depletion Internal medicine medicine Animals Receptor trkB Lymphocyte Count Nerve Growth Factors Rats Wistar Receptor trkA Glucocorticoids Molecular Biology Ecology Evolution Behavior and Systematics Macrophages Epithelial Cells Original Articles Cell Biology Rats Endocrinology nervous system biology.protein Stromal Cells Developmental Biology |
| Zdroj: | Journal of Anatomy. 208:231-238 |
| ISSN: | 1469-7580 0021-8782 |
| DOI: | 10.1111/j.1469-7580.2006.00514.x |
| Popis: | Thymocytes and thymic stromal cells cross-talk in a bidirectional manner within the thymus, thus contributing to the generation of mature T-cells. The thymic stromal cells in the rat express the high- (TrkA, TrkB) and low-affinity (p75NTR) receptors for neurotrophins. In this study we analysed the regulation of TrkA, TrkB and p75NTR expression in the rat thymus by thymocytes. We induced thymocyte apoptosis by administration of corticoids in rats, and then analysed the expression and distribution of these receptors 1, 4 and 10 days later. Thymocyte death was assessed by the activation of caspase-3 in cells undergoing apoptosis. We observed massive thymocyte apoptosis 1 day after injection and, to a lesser extent, after 4 days, which was parallel with a reduction in the density of thymic epithelial cells normally expressing TrkA and p75NTR. Furthermore, TrkA expression was found in cortical thymic epithelial cells, which normally lack this receptor. The expression of TrkB was restricted to a subset of macrophage-dendritic cells, and remained unchanged with treatment. The normal pattern of neurotrophin receptor expression was almost completely restored by day 10. The results demonstrate that the expression of neurotrophin receptors by thymic epithelial cells, but not by macrophage-dendritic cells, is regulated by thymocytes. |
| Databáze: | OpenAIRE |
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