Interactions of the antiviral and antiparkinson agent amantadine with lipid membranes and human erythrocytes
| Autor: | Nathan Dukes, Mariella Altamirano, Fernando Villena, Małgorzata Jemioła-Rzemińska, Kazimierz Strzałka, Mario Suwalsky |
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| Rok vydání: | 2015 |
| Předmět: |
Models
Molecular Erythrocytes Molecular model erythrocyte membrane Lipid Bilayers Biophysics Phospholipid Biochemistry Antiviral Agents Fluorescence spectroscopy Antiparkinson Agents chemistry.chemical_compound Structure-Activity Relationship Differential scanning calorimetry Monolayer Amantadine Humans Lipid bilayer Cell Shape amantadine Dose-Response Relationship Drug Molecular Structure Bilayer Phosphatidylethanolamines Organic Chemistry Cell Membrane Membrane chemistry phospholipid bilayer Microscopy Electron Scanning lipids (amino acids peptides and proteins) Dimyristoylphosphatidylcholine |
| Zdroj: | Biophysical chemistry. 202 |
| ISSN: | 1873-4200 |
| Popis: | Aimed to better understand the molecular mechanisms of its interactions with cell membranes, human erythrocyte and molecular models of the red cell membrane were utilized. The latter consisted of bilayers of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), representative of phospholipid classes located in the outer and inner monolayers of the human erythrocyte membrane, respectively. The capacity of amantadine to perturb the bilayer structures of DMPC and DMPE was evaluated by X-ray diffraction, fluorescence spectroscopy and differential scanning calorimetry (DSC). In an attempt to further elucidate its effects on cell membranes, the present work also examined amantadine influence on the morphology of intact human erythrocytes by means of scanning electron microscopy (SEM). Results indicated that amantadine induced morphological changes to human erythrocytes and interacted in a concentration-dependent manner with DMPC bilayers in contrast to DMPE that was hardly affected by the presence of the drug. |
| Databáze: | OpenAIRE |
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