Prevalence and Detection of Trichomonas vaginalis in HIV-Infected Pregnant Women
| Autor: | Remco P. H. Peters, Lindsey De Vos, Janré Steyn, Jeffrey D. Klausner, Erika Morikawa, Dawie Olivier, Andrew Medina-Marino, Marleen M. Kock, Maanda Mudau, Collin M. Price |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Adult medicine.medical_specialty 030106 microbiology Sexually Transmitted Diseases Trichomonas Infection Trichomonas Infections HIV Infections Dermatology medicine.disease_cause Polymerase Chain Reaction Sensitivity and Specificity World health Article Cohort Studies 03 medical and health sciences South Africa Young Adult 0302 clinical medicine Acquired immunodeficiency syndrome (AIDS) Pregnancy Hiv infected mental disorders Prevalence Trichomonas vaginalis Medicine Humans 030212 general & internal medicine Young adult Pregnancy Complications Infectious Vaginal Smears business.industry Public Health Environmental and Occupational Health Emergency plan medicine.disease Infectious Diseases Family medicine Female business Trichomonas Vaginitis psychological phenomena and processes Cohort study |
| Zdroj: | Sexually transmitted diseases. 45(5) |
| ISSN: | 1537-4521 |
| Popis: | Trichomonas vaginalis is a sexually transmitted infection associated with increased transmission of HIV and significant adverse birth outcomes; culture and polymerase chain reaction (PCR) are commonly used in diagnosis.Consenting HIV-infected pregnant women were recruited from clinics in South Africa and screened for T. vaginalis using PCR. Polymerase chain reaction-positive women provided an additional sample for culture. We compared T. vaginalis detection between PCR and culture, and investigated how PCR cycle threshold (Ct) values differ among culture results.A total of 359 women were enrolled and 76 (20%) tested T. vaginalis PCR positive. Cultures were obtained from 61 of the PCR-positive women, and 38 (62%) were culture positive. The median baseline Ct of the PCR-positive/culture-positive group was 22.6 versus 38.0 among those who were PCR positive/culture negative (P0.001). Culture-positive cases had lower Ct values (higher DNA load); a Ct value less than 30 predicted positivity with a sensitivity of 97% and a specificity of 96%.Culture was positive in roughly half of PCR-positive cases. The culture-negative cases had significantly higher Ct values, indicating a lower concentration of T. vaginalis DNA. A Ct value of 30 provides a reliable threshold for predicting culture positivity. The clinical significance of culture-negative infections detected by PCR is still unclear. |
| Databáze: | OpenAIRE |
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