Distinctive features of positive anti-cell antibody tests (indirect immunofluorescence on HEp-2 cells) in patients with non-autoimmune diseases

Autor:	Silvia Helena Rodrigues, Mônica Simon Prado, H A Mariz, R A Agustinelli, Luiz Eduardo Coelho Andrade
Rok vydání:	2019
Předmět:	Adult Male Anti-nuclear antibody Cell Communicable Diseases Autoimmune Diseases 03 medical and health sciences 0302 clinical medicine Rheumatology Neoplasms Rheumatic Diseases medicine Humans In patient Fluorescent Antibody Technique Indirect 030203 arthritis & rheumatology Indirect immunofluorescence biology business.industry Mental Disorders Autoantibody Middle Aged medicine.anatomical_structure Cross-Sectional Studies 030220 oncology & carcinogenesis Antibodies Antinuclear Immunology biology.protein Female Antibody business
Zdroj:	Lupus. 28(5)
ISSN:	1477-0962
Popis:	Objective The objective of this study was to determine whether characteristics of positive results in the indirect immunofluorescence assay on HEp-2 cells for anti-cell antibodies (HEp-2 IFA) differ between patients with non-autoimmune diseases (NADs) and patients with systemic autoimmune rheumatic diseases (SARDs). Methods Cross-sectional observational study comparing HEp-2 IFA test results in three groups: (a) 558 NAD patients comprising four subgroups (cancer ( n = 95), infectious diseases ( n = 148), psychiatric diseases ( n = 163), common non-infectious chronic diseases ( n = 152)); (b) 194 SARD patients; (c) 1217 healthy individuals (HIs). Sera were tested at 1:80 dilution and diluted to the end titer. Slides were analyzed by two independent blinded examiners. Results A positive HEp-2 IFA test occurred in 102 (18.3%) NAD patients, 170 (87.6%) SARD patients and 150 (12.3%) HIs. The four NAD subgroups did not differ regarding HEp-2 IFA frequency, titer or pattern. HEp-2 IFA titer was higher in NAD patients than in HIs and both had lower titer than SARD patients. Nuclear dense fine speckled pattern was more frequent in NAD patients and HIs than in SARD patients ( p Conclusion Positive HEp-2 IFA frequency, pattern and titer present differential features in NAD and SARD patients, and this attribute adds value to the test in the diagnosis of SARDs.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45fc62428e0fbada4b23ba556dbb6f0b https://pubmed.ncbi.nlm.nih.gov/31027463 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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