Simultaneous determination of zidebactam and cefepime in dog plasma by LC-MS/MS and its application to pre-clinical pharmacokinetic study
| Autor: | Rajesh Chavan, Kiran R. Patil, Ravindra D. Yeole, Mahesh Vithalbhai Patel, Harshad Tambe, V. S. Zope |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Bioanalysis Calibration curve Cefepime 030106 microbiology Clinical Biochemistry Ceftazidime 01 natural sciences Biochemistry Beagle Sensitivity and Specificity Analytical Chemistry 03 medical and health sciences Cyclooctanes Dogs Pharmacokinetics Drug Stability Piperidines Tandem Mass Spectrometry Drug Discovery medicine Protein precipitation Animals Sample preparation Molecular Biology Pharmacology Chromatography Chemistry 010401 analytical chemistry Reproducibility of Results General Medicine 0104 chemical sciences Cephalosporins Linear Models Azabicyclo Compounds medicine.drug Chromatography Liquid |
| Zdroj: | Biomedical chromatography : BMC. 32(8) |
| ISSN: | 1099-0801 |
| Popis: | A precise and accurate liquid chromatography-tandem mass spectrometric (LC-MS/MS) bioanalytical method has been developed and validated for the simultaneous quantification of zidebactam (ZID) and cefepime (FEP) in dog plasma. Ceftazidime was used as an internal standard. Protein precipitation method was used as sample preparation approach. The calibration curve obtained was linear (r ≥ 0.99) over the concentration range 0.156-80 μg/mL for ZID and 0.312-160 μg/mL for FEP. The method was validated as per US Food and Drug Administration guidelines and the results met the acceptance criteria. A run time of 3.5 min for each sample made it possible to analyze the maximum number of samples per day. The proposed method was successfully applied for pharmacokinetic study in beagle dogs. |
| Databáze: | OpenAIRE |
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