Cell‐Free Synthesis of Dopamine and Serotonin in Two Steps with Purified Enzymes
| Autor: | Daniele Rossetto, Graziano Guella, Alessandro Groaz, Ömer Duhan Toparlak, Sheref S. Mansy, Silvia Galvan, Filippo Benedetti, Luca Valer |
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| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
Serotonin Aromatic L-amino acid decarboxylase Cell-Free System Tyrosine hydroxylase Dopamine Biomedical Engineering Tryptophan hydroxylase General Biochemistry Genetics and Molecular Biology Mixed Function Oxygenases Rats Amino acid Biomaterials Monoamine neurotransmitter Enzyme Bacterial Proteins chemistry Biochemistry Aromatic-L-Amino-Acid Decarboxylases medicine Animals Drosophila Proteins medicine.drug |
| Zdroj: | Advanced Biosystems. 4:2000118 |
| ISSN: | 2366-7478 |
| DOI: | 10.1002/adbi.202000118 |
| Popis: | The synthesis of serotonin and dopamine with purified enzymes is described. Both pathways start from an amino acid substrate and synthesize the monoamine neurotransmitter in two enzymatic steps. The enzymes human tryptophan hydroxylase isoform 2, Rattus norvegicus tyrosine hydroxylase, Chlamydia pneumoniae Cpn1046, and aromatic amino acid decarboxylase from Drosophila melanogaster are recombinantly expressed, purified, and shown to be functional in vitro. The hydroxylases efficiently convert L-DOPA (L-dihydroxy-phenylalanine) and 5-HTP (5-hydroxytryptophan) from L-tyrosine and L-tryptophan, respectively. A single aromatic amino acid decarboxylase is capable of converting both hydroxylated intermediates into the final neurotransmitter. The platform described here may facilitate future efforts to generate medically useful artificial cells and nanofactories. |
| Databáze: | OpenAIRE |
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