An Immunohistochemical Survey of SNARE Proteins Shows Distinct Patterns of Expression in Hematolymphoid Neoplasia
| Autor: | Pritish K. Bhattacharyya, Howard Ratech, Rekha Bhat |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Lymphoid Tissue Lymphocyte Biology Plasma cell 03 medical and health sciences Bone Marrow immune system diseases hemic and lymphatic diseases Biomarkers Tumor medicine Humans General Medicine medicine.disease Immunohistochemistry BCL10 Lymphoma Leukemia 030104 developmental biology medicine.anatomical_structure Lymphatic system Membrane protein Tissue Array Analysis Hematologic Neoplasms Bone marrow SNARE Proteins |
| Zdroj: | American Journal of Clinical Pathology. 145:604-616 |
| ISSN: | 1943-7722 0002-9173 |
| DOI: | 10.1093/ajcp/aqw022 |
| Popis: | Objectives: Five proteins from the soluble N -ethylmaleimide–sensitive factor activating protein receptor (SNARE) complex family were studied in normal hematopoietic cells in bone marrow; normal lymphocytes at different stages of maturation and differentiation in bone marrow, thymus, tonsil, and lymph node; malignant lymphomas; and leukemias. Methods: Sixty-eight reactive and 380 hematopoietic and lymphoid neoplasms were immunohistochemically stained for syntaxin 7 (STX7), vesicle-associated membrane proteins (VAMP2, VAMP7, VAMP8), and synaptosomal-associated protein 23 (SNAP23). Results: STX7 has potential for being a useful marker for distinguishing between normal B precursors (hematogones) vs B lymphoblasts, as well as between the “popcorn” cells of nodular lymphocyte-predominant Hodgkin lymphoma vs the Reed-Sternberg cells of classic Hodgkin lymphoma or the B cells of T-cell, histiocyte-rich B-cell lymphoma. VAMP2 is uniquely expressed by both reactive and malignant plasma cells, in contrast to B-cell non-Hodgkin lymphoma. There is differential expression of SNARE proteins in normal and neoplastic lymphoid tissue depending on lymphocyte maturation stage. Conclusions: Differential SNARE protein expression in the lymphoid system may have potential use in diagnosis and may offer clues to lymphoma biology. VAMP2 is a promising new plasma cell marker. |
| Databáze: | OpenAIRE |
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