Diversity and function of the microbial community under strong selective pressure of rotifers
| Autor: | Ariel Marchlewicz, Joanna Starzycka, Beata Klimek, Edyta Fiałkowska, Agnieszka Pajdak-Stós, Aleksandra Walczyńska, Wioleta Kocerba-Soroka |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
microbial decoupling
Microbial Consortia Rotifera Rotifer metabolic activity Applied Microbiology and Biotechnology Pseudomonas mosselii 03 medical and health sciences Animals Food science Phylogeny 030304 developmental biology Trophic level 0303 health sciences biology Bacteria Sewage 030306 microbiology Inoculation General Medicine Biodiversity Nutrients biology.organism_classification Microbial Physiology Culture Media Microbial population biology probiotics Aeration microbial physiology Aeromonas veronii |
| Popis: | We unearthed some interesting microecological discoveries while selecting for the most beneficial bacterial strains to be used as probiotics in Lecane inermis rotifer mass culture. For 3 years, we maintained the cultures of L. inermis, with selection for the highest growth rate and resistance to potential contamination. Then, we conducted further selection and isolation in two groups: rotifers inoculated with the bacterial consortium isolated from the rotifer cultures, and rotifers fed with a commercial bioproduct. Selection was conducted in demanding conditions, with particulate matter suspended in spring water as a substrate, without aeration and under strong consumer pressure, and led to selection of two cultivable strains isolated from the optimal rotifers culture. According to molecular analysis, these strains were Aeromonas veronii and Pseudomonas mosselii. Biolog® ECO plate tests showed that both investigated bacterial communities metabolized wide but similar range of substrates. Therefore, intensely selective conditions led to considerable reduction in bacterial community regarding taxonomy, but not in metabolic activity, showing a functional composition decoupling. Aside from this result, our novel selection method dedicated to the sustainable culture of two trophic levels, a directed selection procedure (DSC), could potentially lead to the development of biotechnologically valuable strains with high metabolic activity and the ability to metabolize different sorts of substrate without harmful impact on higher trophic levels. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|