Naturally Adipose Stromal Cell-Enriched Fat Graft: Comparative Polychromatic Flow Cytometry Study of Fat Harvested by Barbed or Blunt Multihole Cannula
| Autor: | Alessio Caggiati, Antonia Germani, Anna Di Carlo, Giovanna Borsellino, Mario Picozza, Maurizio C. Capogrossi |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Stromal cell medicine.medical_treatment Adipose tissue Transplants Centrifugation Cell Separation 030230 surgery Flow cytometry 03 medical and health sciences 0302 clinical medicine Lipectomy medicine Cannula Humans medicine.diagnostic_test business.industry General Medicine Stromal vascular fraction Middle Aged Flow Cytometry Transplantation Adipose Tissue 030220 oncology & carcinogenesis Liposuction Tissue and Organ Harvesting Surgery Female Stromal Cells business |
| Zdroj: | Aesthetic surgery journal. 37(5) |
| ISSN: | 1527-330X |
| Popis: | Background Fat grafts enriched with cells of the stromal vascular fraction (SVF), especially adipose-derived stromal cells (ASCs), exhibit significantly improved retention over non enriched, plain fat. Different types of liposuction cannulae may yield lipoaspirates with different subpopulations of cells. Moreover, preparation of adipose tissue for transplantation typically involves centrifugation, which creates a density gradient of fat. Objectives The authors sought to determine whether liposuction with a barbed or smooth cannula altered the enrichment of the SVF, and specifically ASCs, in low-density (LD) and high-density (HD) fractions of centrifuged adipose tissue. Methods Fat was harvested from 2 abdominal sites of 5 healthy women with a barbed or smooth multihole blunt-end cannula. After centrifugation, LD and HD fat fractions were digested with collagenase and analyzed by polychromatic flow cytometry to identify and enumerate distinct populations of cells. Results Overall cell yield and the number of immune cells were consistently higher in HD fractions than in LD fractions, regardless of the cannula employed. More living cells, and specifically more ASCs, populated the HD fractions of lipoaspirates obtained with a barbed cannula than with a smooth cannula. Conclusions In this study, lipoaspiration with a barbed cannula and isolation of the HD layer of centrifuged adipose tissue yielded maximal amounts of SVF cells, including ASCs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|