Longikaurin A, a natural ent-kaurane, suppresses stemness in nasopharyngeal carcinoma cells
| Autor: | Xue‑Min Liu, Tian‑Zhu Wang, Yong Du, Mei‑Yuan Liu, Ji Ke Du, Yan Yuan, Xiao‑Ye Hu, Qing Feng Zou, Qian Zhong, Jian-Xin Pu, Hong‑En Yu |
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| Rok vydání: | 2017 |
| Předmět: |
cancer stem cells
0301 basic medicine Cancer Research Cell Biology 03 medical and health sciences 0302 clinical medicine Side population Cancer stem cell Radioresistance otorhinolaryngologic diseases medicine MTT assay longikaurin A nasopharyngeal carcinoma Articles Cell cycle medicine.disease stomatognathic diseases 030104 developmental biology medicine.anatomical_structure Oncology Nasopharyngeal carcinoma Cell culture 030220 oncology & carcinogenesis Cancer research |
| Zdroj: | Oncology Letters |
| ISSN: | 1792-1082 1792-1074 |
| DOI: | 10.3892/ol.2017.5625 |
| Popis: | Cancer stem cells (CSCs) are a small proportion of tumor cells that may be responsible for tumor metastasis and recurrence. Our recent research indicated that longikaurin A (LK-A) exhibited anti-tumor activity in nasopharyngeal carcinoma (NPC) both in vitro and in vivo. Here, we further investigated whether LK-A could suppress the stemness of NPC cells. Sphere formation assay was used to assess the self-renewal ability of the cells treated with LK-A. Side population (SP) was determined by flow cytometry to measure the influence of LK-A on NPC SPs. The expression of the c-myc and fibronectin was detected by western blotting. The cytotoxicity of LK-A in combination with cisplatin to NPC cells was determined by MTT assay. Colony formation assay was used to verify whether LK-A could sensitize NPC cells to radiation and reverse the radiotherapy resistance. In the present study, we found that LK-A reduced the number and size of spheroid formation and decreased the SP cell percentage of the S18 cell line at a low concentration. Furthermore, LK-A treatment downregulated the expression of c-myc and fibronectin in NPC cell lines. Moreover, LK-A could significantly enhance the chemotherapeutic and radiotherapeutic sensitivity of NPC cell lines and reverse acquired radiotherapy resistance of Sune2-IR. Our data revealed that LK-A could suppress the stemness of NPC cells and may enhance the efficacy of radiotherapy and chemotherapy. |
| Databáze: | OpenAIRE |
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