Calicivirus intracellular RNA: Fractionation of 18–22 S RNA and lack of typical 5′-methylated caps on 36 S and 22 S San Miguel sea lion virus RNAs
| Autor: | Douglas W. Ehresmann, Frederick L. Schaffer |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
viruses
Calicivirus RNA-dependent RNA polymerase RNA Biology Methylation Virology Molecular biology Caniformia chemistry.chemical_compound RNA silencing Biosynthesis chemistry Centrifugation Density Gradient Vero cell Animals Nucleic Acid Conformation RNA Viral Caliciviridae Polyacrylamide gel electrophoresis Subgenomic mRNA |
| Zdroj: | Virology. 95:251-255 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/0042-6822(79)90426-4 |
| Popis: | Radiolabeled RNA from Vero cells infected with a calicivirus, San Miguel sea lion virus type 2, in the presence of actinomycin D, was fractionated by glycerol density gradient sedimentation to yield 36 S single-stranded RNA (ssRNA) and heterogenous RNA sedimenting in the 18–22 S region. Further fractionation of 18–22 S RNA by LiCl precipitation, CF-11 cellulose chromatography, and polyacrylamide gel electrophoresis yielded 22 S ssRNA, two doule-stranded RNAs )dsRNA), and a partially single-partially double-stranded RNA (ss/dsRNA). Sedimentation analysis indicated the major and minor dsRNAs were equivalent to two strands of 36 S and 22 S ssRNAs, respectively, and the dsRNA core of ss/dsRNA was equivalent to the major dsRNA. Neither 22 S ssRNA (presumed subgenomic mRNA) nor 36 S ssRNA (presummed full genomic RNA plus intracellular virion RNA) had the typical 5′-methylated cap. |
| Databáze: | OpenAIRE |
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