DNA tetrahedral scaffolds-based platform for the construction of electrochemiluminescence biosensor
| Autor: | Hong-Yuan Chen, Qiu-Mei Feng, Jing-Juan Xu, Meixing Li, Wei Zhao, Zhen Zhou |
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| Rok vydání: | 2017 |
| Předmět: |
Materials science
Immobilized Nucleic Acids Biomedical Engineering Biophysics Metal Nanoparticles Nanotechnology Biosensing Techniques 02 engineering and technology 010402 general chemistry 01 natural sciences Luminol chemistry.chemical_compound Cell Line Tumor Neoplasms Quantum Dots Cadmium Compounds Electrochemistry Humans Electrochemiluminescence Surface plasmon resonance Selenium Compounds Telomerase Luminescent Agents General Medicine Surface Plasmon Resonance 021001 nanoscience & nanotechnology Nanostructures 0104 chemical sciences Förster resonance energy transfer chemistry Colloidal gold Luminescent Measurements Electrode Gold 0210 nano-technology Biosensor DNA HeLa Cells Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 90:251-257 |
| ISSN: | 0956-5663 |
| Popis: | Proximal metallic nanoparticles (NPs) could quench the electrochemiluminescence (ECL) emission of semiconductor quantum dots (QDs) due to Förster energy transfer (FRET), but at a certain distance, the coupling of light-emission with surface plasmon resonance (SPR) result in enhanced ECL. Thus, the modification strategies and distances control between QDs and metallic NPs are critical for the ECL intensity of QDs. In this strategy, a SPR enhanced ECL sensor based on DNA tetrahedral scaffolds modified platform was reported for the detection of telomerase activity. Due to the rigid three-dimensional structure, DNA tetrahedral scaffolds grafting on the electrode surface could accurately modulate the distance between CdS QDs and luminol labelled gold nanoparticles (L-Au NPs), meanwhile provide an enhanced spatial dimension and accessibility for the assembly of multiple L-Au NPs. The ECL intensities of both CdS QDs (-1.25V vs. SCE) and luminol (+0.33V vs. SCE) gradually increased along with the formation of multiple L-Au NPs at the vertex of DNA tetrahedral scaffolds induced by telomerase, bringing in a dual-potential ECL analysis. The proposed method showed high sensitivity for the identification of telomerase and was successfully applied for the differentiation of cancer cells from normal cells. This work suggests that DNA tetrahedral scaffolds could serve as an excellent choice for the construction of SPR-ECL system. |
| Databáze: | OpenAIRE |
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