Cytosine-phosphate-guanine oligodeoxynucleotides containing GACGTT motifs enhance the immune responses elicited by keyhole limpet hemocyanin antigen in dairy cattle
| Autor: | Chun-Yen Chu, Chi Yu, Shyh-Shyan Liu, Yu-Ming Lin, Shang-Chun Lee, Kuo-Hua Lee, Xin Zhao, Jai-Wei Lee, Perng-Chi Shen |
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| Rok vydání: | 2011 |
| Předmět: |
CpG Oligodeoxynucleotide
Guanine Freund's Adjuvant Gene Expression Biology Biochemistry chemistry.chemical_compound Interferon-gamma Immune system Antigen Adjuvants Immunologic Pregnancy Drug Discovery Genetics Animals Lymphocytes Antigens Molecular Biology Cell Proliferation Base Sequence Immune Sera Acquired immune system Molecular biology Toll-Like Receptor 9 Milk CpG site chemistry Immunoglobulin M Oligodeoxyribonucleotides Immunoglobulin G Hemocyanins biology.protein Leukocytes Mononuclear Molecular Medicine Cytokines Cattle Female Keyhole limpet hemocyanin Dinucleoside Phosphates |
| Zdroj: | Nucleic acid therapeutics. 21(5) |
| ISSN: | 2159-3345 |
| Popis: | Adjuvants are important components of vaccine formulations. Effective adjuvants line innate and adaptive immunity by signaling through pathogen recognition receptors. Synthetic cytosine-phosphate-guanine (CpG) oligodeoxynucleotides (ODNs) have been shown to have potentials as adjuvants for vaccines. However, the immunostimulatory effect of CpG is species-specific and depends on the sequence of CpG motifs. A CpG ODN (2135), containing 3 identical copies of GTCGTT motif, was previously reported to have the strongest effects on bovine peripheral blood mononuclear cells (PBMC). Based on the sequence of 2135, we replaced the GTCGTT motif with 11 other sequences containing CG and investigated their effects on bovine lymphocyte proliferation. Results showed that the CpG ODNs containing 3 copies of GACGTT motif had the highest lymphocyte stimulation index (7.91±1.18), which was significantly (P0.05) higher than that of 2135 (4.25±0.56). The CpG ODNs containing 3 copies of GACGTT motif also significantly increased the mRNA expression of interferon (IFN)-α, interleukin (IL)-12, and IL-21 in bovine PBMC. When dairy cows were immunized with the keyhole limpet hemocyanin (KLH) antigen formulated with CpG ODNs containing 3 copies of GACGTT, production of KLH-specific antibodies in serum and in milk whey was significantly (P0.05) enhanced. IFN-γ in whole blood stimulated by KLH was also significantly (P0.05) increased in cows immunized with KLH plus CpG ODNs. Our results indicate that CpG ODNs containing 3 copies of the GACGTT motifs is a potential adjuvant for bovine vaccines. |
| Databáze: | OpenAIRE |
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