Effects of electrode surface modification with chlorotoxin on patterning single glioma cells
| Autor: | Omid Veiseh, Jian Xu, Xiaohao Zheng, Fareid Asphahani, Fumio S. Ohuchi, Miqin Zhang, Myo Thein |
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| Rok vydání: | 2011 |
| Předmět: |
Surface Properties
General Physics and Astronomy Scorpion Venoms Peptide Nanotechnology Article chemistry.chemical_compound Cell Adhesion Tumor Cells Cultured Animals Physical and Theoretical Chemistry Cell adhesion chemistry.chemical_classification Adhesion Glioma Rats Microelectrode Chlorotoxin chemistry Covalent bond Tissue Array Analysis Cancer cell Biophysics Surface modification Adsorption Gold Microelectrodes |
| Zdroj: | Physical chemistry chemical physics : PCCP. 13(19) |
| ISSN: | 1463-9084 |
| Popis: | A microchip patterned with arrays of single cancer cells can be an effective platform for the study of tumor biology, medical diagnostics, and drug screening. However, patterning and retaining viable single cancer cells on defined sites of the microarray can be challenging. In this study we used a tumor cell-specific peptide, chlorotoxin (CTX), to mediate glioma cell adhesion on arrays of gold microelectrodes and investigated the effects of three surface modification schemes for conjugation of CTX to the microelectrodes on single cell patterning, which include physical adsorption, covalent bonding mediated by N-hydroxysuccinimide (NHS), and covalent bonding via crosslinking succinimidyl iodoacetate and Traut's (SIA-Traut) reagents. The CTX immobilization to microelectrodes was confirmed by high-resolution X-ray photoelectron spectroscopy. Physically adsorbed CTX showed better support for cell adhesion and is more effective in confining adhered cells on the electrodes than covalently-bound CTX. Furthermore, cell adhesion and spreading on microelectrodes were quantified in real-time by impedance measurements, which revealed an impedance signal from physically adsorbed CTX electrodes four times greater than the signal from covalently-bound CTX electrodes. |
| Databáze: | OpenAIRE |
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