Metal-ion Assisted, Radiation-induced Conversion of B- to Z-DNA in Poly (dGdC) and Two Natural DNAs
| Autor: | J.K. Masselink, C K Hyde, Y N Myers, R.D. Hudson, C N Rhodes, Conrad N. Trumbore |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
chemistry.chemical_classification
Purine Circular dichroism Radiological and Ultrasound Technology Pyrimidine Base (chemistry) Stereochemistry Radiation induced DNA Metal Z-DNA chemistry.chemical_compound Polydeoxyribonucleotides chemistry Gamma Rays Metals visual_art visual_art.visual_art_medium Biophysics Nucleic Acid Conformation Radiology Nuclear Medicine and imaging |
| Zdroj: | International Journal of Radiation Biology. 66:479-483 |
| ISSN: | 1362-3095 0955-3002 |
| DOI: | 10.1080/09553009414551491 |
| Popis: | Metal-ion assisted, gamma-radiation-induced B-Z conformation changes have been observed with UV and circular dichroism spectroscopy for poly (dGdC), calf thymus and herring testis DNA. These conformational changes are similar to those induced by increasing multivalent metal ion concentrations in DNA containing alternating purine and pyrimidine base sequences. In both the metal-ion-induced and the metal-ion-assisted, radiation-induced conformation changes, the conversions were from the right-handed B-DNA to the left-handed Z-DNA conformation. It is proposed that radiation-induced DNA strand breaks markedly reduce the high activation energy barrier in the metal ion-driven B-Z conformation conversion and allow much smaller metal ion concentrations to induce this conversion than in the absence of strand breaks. The biological importance of such radiation-induced conformational changes is discussed in terms of the potential significance of the Z-DNA conformation in the control of the DNA transcription process. |
| Databáze: | OpenAIRE |
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