Interleukin 8 (IL-8) Induces the Expression of Kinin B1Receptor in Human Lung Fibroblasts
| Autor: | Jean-Luc Paquet, S. Bastian, Cremers B, François Marceau, Bruno Loillier, Dimcho Bachvarov, Jean-François Larrivée, Robert C, Didier Pruneau |
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| Rok vydání: | 1998 |
| Předmět: |
Chemokine
medicine.medical_specialty Receptor expression Biophysics Receptor Bradykinin B1 Biochemistry Antibodies Cell Line Receptors Interleukin-8A Antigens CD Internal medicine medicine Humans RNA Messenger Northern blot Interleukin 8 Receptor Lung Molecular Biology Messenger RNA biology Chemistry Receptors Bradykinin Interleukin-8 Receptors Interleukin Cell Biology Fibroblasts Kallidin Kinin Endocrinology biology.protein Antibody Interleukin-1 Protein Binding |
| Zdroj: | Biochemical and Biophysical Research Communications. 253:750-755 |
| ISSN: | 0006-291X |
| Popis: | Kinin B 1 receptors are induced by various inflammatory mediators. The aim of the present study was to investigate the effect of the CXC chemokine IL-8 on kinin B 1 receptor expression in IMR-90 cells, by performing binding studies and Northern blot analysis of B 1 receptor mRNA levels. We demonstrated here that the density of the kinin B 1 receptors could be increased by the chemokine IL-8 in a concentration- and time-dependent manner. IL-8 also increased the kinin B 1 receptor mRNA level in IMR-90 cells. IL-8-induced B 1 receptor expression could be totally abolished by pretreatment with the metabolic inhibitors. Furthermore, expression was markedly reduced by antibodies to human IL-1β. In conclusion, IL-8 increased the expression of kinin B 1 receptors in IMR-90 cells and this effect is likely to be secondary to the production of IL-1β. |
| Databáze: | OpenAIRE |
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