Monodisperse Sequence-Controlled α-l-Fucosylated Glycooligomers and Their Multivalent Inhibitory Effects on LecB
| Autor: | Filip Kovacic, Nikolina Babic, Laura Hartmann, Tanja Freichel, Katharina Susanne Bücher |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Glycosylation
Polymers and Plastics Oligosaccharides Bioengineering 010402 general chemistry medicine.disease_cause Ligands 01 natural sciences Fucose Bacterial Adhesion Biomaterials chemistry.chemical_compound Solid-phase synthesis Glycomimetic Lectins Materials Chemistry medicine Surface plasmon resonance biology 010405 organic chemistry Pseudomonas aeruginosa Biofilm Lectin biology.organism_classification 0104 chemical sciences Anti-Bacterial Agents chemistry Biochemistry Carbohydrate Sequence Biofilms biology.protein Blood Group Antigens Bacteria Biotechnology Protein Binding |
| Zdroj: | Macromolecular bioscience. 18(12) |
| ISSN: | 1616-5195 |
| Popis: | The opportunistic bacterium Pseudomonas aeruginosa, often exhibiting multiresistance against conventional antibiotics, expresses the lectin LecB that is suspected to be an important factor during biofilm formation via interactions with cell-surface presented carbohydrate ligands such as the blood group antigens. Therefore, carbohydrate-based ligands interfering with LecB binding have the potential to lead to new anti-biofilm and anti-adhesion therapies. This study explores in vitro binding potencies of glycomimetic ligands containing up to six α-l-fucose ligands on a monodisperse, sequence-controlled oligoamide scaffold interacting with LecB. Surface plasmon resonance (SPR) and a modified enzyme-linked lectin assay (mELLA) revealed an increasing affinity to LecB with increasing fucose valency. Furthermore, fucosylated glycooligomers were shown to inhibit the formation of P. aeruginosa biofilm up to 20%. Overall these results show the potential of fucosylated oligoamides to be further developed as inhibitors of LecB binding and biofilm formation. |
| Databáze: | OpenAIRE |
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