Repetitive high-frequency transcranial magnetic stimulation reverses depressive-like behaviors and protein expression at hippocampal synapses in chronic unpredictable stress-treated rats by enhancing endocannabinoid signaling
| Autor: | Quan-rui Ma, Fen Xue, Cui-hong Zhou, Shiquan Wang, Ying Wang, Huaning Wang, Shan-shan Xue, Qingrong Tan, Zhengwu Peng |
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| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_treatment Clinical Biochemistry Hippocampus Stimulation Hippocampal formation Toxicology Biochemistry Rats Sprague-Dawley 03 medical and health sciences Behavioral Neuroscience 0302 clinical medicine Receptor Cannabinoid CB1 Pregnancy Stress Physiological medicine Animals Humans Receptor Biological Psychiatry Pharmacology Neurons Gene knockdown Behavior Animal Chemistry Depression musculoskeletal neural and ocular physiology Endocannabinoid system Transcranial Magnetic Stimulation 030227 psychiatry Rats Transcranial magnetic stimulation Lipoprotein Lipase nervous system Animals Newborn Gene Expression Regulation Astrocytes Gene Knockdown Techniques Synapses Female Cannabinoid Neuroscience 030217 neurology & neurosurgery Endocannabinoids |
| Zdroj: | Pharmacology, biochemistry, and behavior. 184 |
| ISSN: | 1873-5177 |
| Popis: | The anti-depressant effect of repetitive transcranial magnetic stimulation (rTMS), a clinically-useful treatment for depression, is associated with changes to the endocannabinoid system (ECS). However, it is currently unknown whether different frequencies of rTMS alter the ECS differently. To test this, rats exposed to chronic unpredictable stress (CUS) were treated with rTMS at two different frequencies (5 (high) or 1 Hz (low), 1.26 Tesla) for 7 consecutive days. Twenty-four hours after the final rTMS treatment, we evaluated depressive-like behaviors and the expression of several synaptic proteins and ECS-related proteins in the hippocampus. In addition, we knocked-down diacylglycerol lipase alpha (DAGLα) and cannabinoid type 1 receptor (CB1R), two important components of the ECS, and measured depressive-like behaviors and synaptic protein expression following rTMS. Furthermore, we measured the expression levels of several components of the ECS system in hippocampal-derived astrocytes and neurons exposed to repetitive magnetic stimulation (rMS) with different parameters (5 or 1 Hz, 0.84 or 1.26 Tesla). Interestingly, we found that only high-frequency rTMS ameliorated depressive-like behaviors and normalized the expression of hippocampal synaptic proteins in CUS-treated rats; this effect was eliminated by knockdown of DAGLα or CB1R. Moreover, we found that rMS at 5 Hz increased the expression of DAGLα and CB1R in hippocampal astrocytes and neurons. Collectively, our results suggest that high-frequency rTMS exerts its anti-depressant effect by up-regulating DAGLα and CB1R. |
| Databáze: | OpenAIRE |
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