Pulmonary and pleural inflammation after intratracheal instillation of short single-walled and multi-walled carbon nanotubes
| Autor: | Ayako Nakamura, Kanako Uchino, Naohide Shinohara, Shigehisa Endoh, Junko Maru, Kazumasa Honda, Haruhisa Kato, Katsuhide Fujita, Makiko Fukuda |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty Pleural inflammation Time Factors Intratracheal instillation Inflammation 02 engineering and technology Carbon nanotube Toxicology law.invention Lymphatic System 03 medical and health sciences law medicine Animals Rats Wistar Single-walled carbon nanotubes (SWCNTs) Lung Pleurisy Multi-walled carbon nanotubes (MWCNTs) Inhalation Exposure medicine.diagnostic_test Nanotubes Carbon Chemistry Gene Expression Profiling Pulmonary inflammation Pneumonia General Medicine 021001 nanoscience & nanotechnology Surgery 030104 developmental biology Bronchoalveolar lavage Lymphatic system Cytokines Pleura Lymph Inflammation Mediators medicine.symptom 0210 nano-technology |
| Zdroj: | Toxicology Letters. 257:23-37 |
| ISSN: | 0378-4274 |
| DOI: | 10.1016/j.toxlet.2016.05.025 |
| Popis: | Relationships between the physical properties of carbon nanotubes (CNTs) and their toxicities have been studied. However, little research has been conducted to investigate the pulmonary and pleural inflammation caused by short-fiber single-walled CNTs (SWCNTs) and multi-walled CNTs (MWCNTs). This study was performed to characterize differences in rat pulmonary and pleural inflammation caused by intratracheal instillation with doses of 0.15 or 1.5mg/kg of either short-sized SWCNTs or MWCNTs. Data from bronchoalveolar lavage fluid analysis, histopathological findings, and transcriptional profiling of rat lungs obtained over a 90-day period indicated that short SWCNTs caused persistent pulmonary inflammation. In addition, the short MWCNTs markedly impacted alveoli immediately after instillation, with the levels of pulmonary inflammation following MWCNT instillation being reduced in a time-dependent manner. MWCNT instillation induced greater levels of pleural inflammation than did short SWCNTs. SWCNTs and MWCNTs translocated in mediastinal lymph nodes were observed, suggesting that SWCNTs and MWCNTs underwent lymphatic drainage to the mediastinal lymph nodes after pleural penetration. Our results suggest that short SWCNTs and MWCNTs induced pulmonary and pleural inflammation and that they might be transported throughout the body after intratracheal instillation. The extent of changes in inflammation differed following SWCNT and MWCNT instillation in a time-dependent manner. |
| Databáze: | OpenAIRE |
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