Antibacterial activity of novel peptide derived from Cry1Ab16 toxin and development of LbL films for foodborne pathogens control
| Autor: | Cristina Delerue-Matos, Peter Eaton, Alexandra Plácido, Krystallenia Batziou, Idalina Bragança, Alyne Rodrigues de Araújo, Mariela M. Marani, José Roberto S.A. Almeida Leite, Valentina F. Domingues, Andreanne G. Vasconcelos |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Materials science Antimicrobial peptides Bacillus thuringiensis Bioengineering Peptide 02 engineering and technology medicine.disease_cause Microbiology Biomaterials Foodborne Diseases 03 medical and health sciences Minimum inhibitory concentration Hemolysin Proteins Bacterial Proteins medicine Escherichia coli chemistry.chemical_classification Minimum bactericidal concentration biology Bacillus thuringiensis Toxins Toxin Membranes Artificial 021001 nanoscience & nanotechnology biology.organism_classification Anti-Bacterial Agents Endotoxins 030104 developmental biology chemistry Mechanics of Materials 0210 nano-technology Antibacterial activity Peptides Bacteria |
| Zdroj: | Materials scienceengineering. C, Materials for biological applications. 75 |
| ISSN: | 1873-0191 |
| Popis: | Escherichia coli is one of the most common etiological agents of diarrhea in developing countries. The appearance of resistant E. coli prevents treatment of these infections. Biotechnological products incorporating antimicrobial peptides are currently being considered in applications to prevent intestinal infections by these bacteria. The aim of this study was to evaluate the antibacterial activity of the peptide PcL342-354C, which is derived from the toxin Cry1Ab16 from Bacillus thuringiensis, against E. coli strains. We also report the preparation, characterization and evaluation of the antibacterial activity of LbL films containing PcL342-354C. The results showed that the PcL342-354C peptide inhibited the growth of different strains of E. coli with minimal inhibitory concentration ranging from 15.62–31.25 μg/mL and minimal bactericidal concentration was 250 μg/mL, indicating a potential antibacterial activity. The morphology of an ITO/Cashew gum/PcL342-354C film was analysed using atomic force microscopy which showed an increase of roughness due to the increase in the number of layers. The LbL films showed significant antibacterial activity against E. coli NCTC 9001 in both conditions tested (10 and 20 bilayers). Our results indicate that the peptide exhibits an antibacterial potential that can be tapped to develop biomaterials with antibacterial activity for use against foodborne pathogens. |
| Databáze: | OpenAIRE |
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