The effects of transplanted mesenchymal stem cells treated with 17-b estradiol on experimental autoimmune encephalomyelitis

Autor: Soudeh Khanamani Falahati-Pour, Gholamhossein Hassanshahi, Ayat Kaeidi, Fateme Asadi, Seyyed Meysam Abtahi Froushani, Mohammad Reza Mirzaei, Rahim Heidari barchi nezhad, Zahra Hashemi
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
medicine.medical_specialty
Encephalomyelitis
Autoimmune
Experimental
Spleen
Mesenchymal Stem Cell Transplantation
MMP8
Proinflammatory cytokine
03 medical and health sciences
0302 clinical medicine
Immune system
Osteogenesis
T-Lymphocyte Subsets
Internal medicine
Genetics
Animals
Medicine
Lymphocytes
RNA
Messenger
Molecular Biology
Cell Proliferation
Adipogenesis
Estradiol
business.industry
Multiple sclerosis
Body Weight
Mesenchymal stem cell
Experimental autoimmune encephalomyelitis
Cell Differentiation
Mesenchymal Stem Cells
General Medicine
medicine.disease
Matrix Metalloproteinases
Rats
Transplantation
Disease Models
Animal
030104 developmental biology
medicine.anatomical_structure
Endocrinology
030220 oncology & carcinogenesis
Cytokines
Inflammation Mediators
business
Zdroj: Molecular Biology Reports. 46:6135-6146
ISSN: 1573-4978
0301-4851
Popis: The present study was conducted aimed at exploring the modulatory effects of 17-b estradiol (17-bED) on mesenchymal stem cells (MSCs) in the EAE (experimental autoimmune encephalomyelitis) animal model of multiple sclerosis (MS). Following the isolation of bone marrow-derived MSCs from the bilateral femurs and tibias of the male Wistar rats, the cells were harvested and cultured in the presence of 100 nM 17-bED for 24 h. EAE was induced in male Wistar rats (8-12 weeks old) using guinea pig spinal cord homogenate, in combination with the complete Freund's adjuvant. The MSC therapy was triggered when all of the animals obtained a disability score. The symptoms were monitored on a daily basis throughout the study until the rats were euthanized. The mRNA expression of cytokines, including IL-17, IFN-γ, TNF-α, IL-10, IL-4, and TGF-β together with MMP8 and MMP9 as the family members of matrix metalloproteinases (MMPs) in the brain and spinal cord tissues were examined using real-time PCR. The levels of splenocytes-originated IL-10 and IFN-γ cytokines were also measured by ELISA. The MTT-based research findings showed that the infiltration of lymphocytes into the spleen decreased considerably. It was also observed that the mRNA expression of proinflammatory cytokines decreased significantly, while the mRNA levels of anti-inflammatory cytokines increased remarkably. It was also found that the mRNA levels of the examined matrix metalloproteinases (MMP8 and MMP9) were downregulated significantly. The findings of the present study indicated that the administration of 17-bED enhanced the efficacy of MSCs transplantation and modulated immune responses relatively in the EAE model, via the regulation of either pro- or anti-inflammatory cytokines and matrix metalloproteinases.
Databáze: OpenAIRE
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