The DNA-binding network of Mycobacterium tuberculosi s
| Autor: | Nathan D. Price, Jessica K. Winkler, Nitin S. Baliga, Chris Mawhinney, David J Reiss, Eliza J. R. Peterson, Shuyi Ma, David R. Sherman, Bob Morrison, Serdar Turkarslan, James E. Galagan, Tige R. Rustad, Mark J. Hickey, William Brabant, Kyle J. Minch |
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| Rok vydání: | 2015 |
| Předmět: |
DNA
Bacterial Chromatin Immunoprecipitation Transcription Genetic Amino Acid Motifs Genetic Vectors General Physics and Astronomy DNA-binding protein Article General Biochemistry Genetics and Molecular Biology law.invention Microbiology Mycobacterium tuberculosis chemistry.chemical_compound Bacterial Proteins Transcription (biology) law Nucleotide Motifs Binding site Promoter Regions Genetic Transcription factor Genetics Binding Sites Multidisciplinary biology Gene Expression Profiling Computational Biology Gene Expression Regulation Bacterial General Chemistry biology.organism_classification Recombinant Proteins 3. Good health DNA-Binding Proteins ROC Curve chemistry Recombinant DNA Chromatin immunoprecipitation DNA Genome-Wide Association Study Protein Binding Transcription Factors |
| Zdroj: | Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Mycobacterium tuberculosis (MTB) infects 30% of all humans and kills someone every 20–30 s. Here we report genome-wide binding for ~80% of all predicted MTB transcription factors (TFs), and assayed global expression following induction of each TF. The MTB DNA-binding network consists of ~16,000 binding events from 154 TFs. We identify >50 TF-DNA consensus motifs and >1,150 promoter-binding events directly associated with proximal gene regulation. An additional ~4,200 binding events are in promoter windows and represent strong candidates for direct transcriptional regulation under appropriate environmental conditions. However, we also identify >10,000 ‘dormant’ DNA-binding events that cannot be linked directly with proximal transcriptional control, suggesting that widespread DNA binding may be a common feature that should be considered when developing global models of coordinated gene expression. Adaptation of Mycobacterium tuberculosis to the host environment is principally mediated through its transcription factors. Here, the authors report the DNA binding and transcriptional profile of ~80% of all predicted M. tuberculosis transcription factors, and find wide-spread dormant DNA binding. |
| Databáze: | OpenAIRE |
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