c-Cbl shRNA-expressing adenovirus sensitizes TRAIL-induced apoptosis in prostate cancer DU-145 through increases of DR4/5
| Autor: | Jae J. Song, Joo Hang Kim, Sangah Kim |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Cancer Research Genetic Vectors Apoptosis Biology medicine.disease_cause Adenoviridae TNF-Related Apoptosis-Inducing Ligand Small hairpin RNA Downregulation and upregulation In vivo Cell Line Tumor hemic and lymphatic diseases medicine Humans Proto-Oncogene Proteins c-cbl RNA Small Interfering Receptor Molecular Biology fungi Prostatic Neoplasms Genetic Therapy Molecular biology Receptors TNF-Related Apoptosis-Inducing Ligand Cell culture Molecular Medicine Tumor necrosis factor alpha |
| Zdroj: | Cancer Gene Therapy. 20:82-87 |
| ISSN: | 1476-5500 0929-1903 |
| Popis: | We previously demonstrated that the downregulation of Casitas B-lineage lymphoma (c-Cbl) can sensitize tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in two different ways. One way is to block the rapid degradation of TRAIL receptors, which can sustain TRAIL-induced apoptosis for a long time. Here, we designed a replication-defective adenovirus expressing the short hairpin RNA (shRNA) against c-Cbl to test the possibility of developing a cancer gene therapy that can act as a sensitizer of TRAIL. As expected from the results of our previous study that used a stable cell line with downregulated c-Cbl, infection with the c-Cbl shRNA-expressing adenovirus led to an increase in the death receptor 4 (DR4) and DR5 levels, which is known to be a cause for the increase of TRAIL-induced apoptosis. In conclusion, we demonstrated that c-Cbl shRNA-expressing adenovirus is able to sensitize TRAIL-induced apoptosis in vivo as well as in vitro. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|