Inhibition of axonal growth by SNAP-25 antisense oligonucleotides in vitro and in vivo
Autor: | Marina Catsicas, Gabriele Grenningloh, Stefan Catsicas, Guidon Ayala, Astrid Osen-Sand, Kenneth Jones, Jonathan Knowles, Julie K. Staple |
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Rok vydání: | 1993 |
Předmět: |
Nervous system
Synaptosomal-Associated Protein 25 Neurite Molecular Sequence Data Nerve Tissue Proteins Chick Embryo Biology PC12 Cells Retina In vivo Neurites medicine Animals Nerve Growth Factors Axon Growth cone Cells Cultured Neurons Multidisciplinary Base Sequence Membrane Proteins Anatomy Oligonucleotides Antisense Thionucleotides Axons Rats Cell biology medicine.anatomical_structure Nerve growth factor Animals Newborn nervous system Neuron |
Zdroj: | Nature. 364:445-448 |
ISSN: | 1476-4687 0028-0836 |
Popis: | Axonal elongation and the transformation of growth cones to synaptic terminals are major steps of brain development and the molecular mechanisms involved form the basis of the correct wiring of the nervous system. The same mechanisms may also contribute to the remodelling of nerve terminals that occurs in the adult brain, as a morphological substrate to memory and learning. We have investigated the function of the nerve terminal protein SNAP-25 (ref. 2) during development. We report here that SNAP-25 is expressed in axonal growth cones during late stages of elongation and that selective inhibition of SNAP-25 expression prevents neurite elongation by rat cortical neurons and PC-12 cells in vitro and by amacrine cells of the developing chick retina in vivo. These results demonstrate that SNAP-25 plays a key role in axonal growth. They also suggest that high levels of SNAP-25 expression in specific areas of the adult brain may contribute to nerve terminal plasticity. |
Databáze: | OpenAIRE |
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