Differential metabolism-associated gene expression of duck pancreatic cells in response to two strains of duck hepatitis A virus type 1
| Autor: | Fu Guanghua, Liu Rongchang, Cui-Qin Huang, Fu Qiuling, Shi Shaohua, Huang Yu, Chen Zhen, Chen Hongmei, Chunhe Wan, Cheng Longfei |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Gene Expression
Biology Real-Time Polymerase Chain Reaction Deep sequencing Hepatitis Virus Duck Microbiology Serine Transcriptome Virology Gene expression Animals Phosphoserine Aminotransferase Amino Acids Pancreas Poultry Diseases Picornaviridae Infections cDNA library Sequence Analysis RNA Phosphoserine phosphatase General Medicine Metabolism disorder Ducks Pancreatitis Hepatitis Viral Animal Host-Pathogen Interactions Original Article |
| Zdroj: | Archives of Virology |
| ISSN: | 1432-8798 0304-8608 |
| Popis: | Several outbreaks of duck hepatitis A virus type 1 (DHAV-1), which were characterized by yellow coloration and hemorrhage in pancreatic tissues, have occurred in China. The causative agent is called pancreatitis-associated DHAV-1. The mechanisms involved in pancreatitis-associated DHAV-1 infection are still unclear. Transcriptome analysis of duck pancreas infected with classical-type DHAV-1 and pancreatitis-associated DHAV-1 was carried out. Deep sequencing with Illumina-Solexa resulted in a total of 53.9 Gb of clean data from the cDNA library of the pancreas, and a total of 29,597 unigenes with an average length of 993.43 bp were generated by de novo sequence assembly. The expression levels of D-3-phosphoglycerate dehydrogenase, phosphoserine aminotransferase, and phosphoserine phosphatase, which are involved in glycine, serine, and threonine metabolism pathways, were significantly downregulated in ducks infected with pancreatitis-associated DHAV-1 compared with those infected with classical-type DHAV-1. These findings provide information regarding differences in expression levels of metabolism-associated genes between ducks infected with pancreatitis-associated DHAV-1 and those infected with classical-type DHAV-1, indicating that intensive metabolism disorders may contribute to the different phenotypes of DHAV-1-infection. |
| Databáze: | OpenAIRE |
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