Amoxicillin-Clavulanic Acid Resistance in the Genus Bifidobacterium
| Autor: | Alice Viappiani, Leonardo Mancabelli, Sergio Bernasconi, Giulia Longhi, Walter Mancino, Francesca Turroni, Gabriele Andrea Lugli, Marco Ventura, Douwe van Sinderen, Christian Milani, Rosaria Anzalone, Chiara Argentini |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
musculoskeletal diseases
medicine.drug_class Antibiotics ved/biology.organism_classification_rank.species Gut flora Amoxicillin-Potassium Clavulanate Combination digestive system Applied Microbiology and Biotechnology Microbial Ecology Microbiology 03 medical and health sciences Drug Resistance Bacterial medicine Humans Child Gene 030304 developmental biology Bifidobacterium 0303 health sciences Amoxicillin/clavulanic acid Bifidobacterium breve Ecology biology 030306 microbiology ved/biology Infant Antimicrobial biology.organism_classification Isolation (microbiology) Anti-Bacterial Agents Gastrointestinal Microbiome Child Preschool Food Science Biotechnology medicine.drug |
| Zdroj: | Appl Environ Microbiol |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.03137-20 |
| Popis: | Amoxicillin-clavulanic acid (AMC) is one of the most frequently prescribed antibiotic formulations in the Western world. Extensive oral use of this antimicrobial combination influences the gut microbiota. One of the most abundant early colonizers of the human gut microbiota is represented by different taxa of the Bifidobacterium genus, which include many members that are considered to bestow beneficial effects upon their host. In the current study, we investigated the impact of AMC administration on the gut microbiota composition, comparing the gut microbiota of 23 children that had undergone AMC antibiotic therapy to that of 19 children that had not been treated with antibiotics during the preceding 6 months. Moreover, we evaluated AMC sensitivity by MIC test of 261 bifidobacterial strains, including reference strains for the currently recognized 64 bifidobacterial (sub)species, as well as 197 bifidobacterial isolates of human origin. These assessments allowed the identification of four bifidobacterial strains that exhibit a high level of AMC insensitivity, which were subjected to genomic and transcriptomic analyses to identify the putative genetic determinants responsible for this AMC insensitivity. Furthermore, we investigated the ecological role of AMC-resistant bifidobacterial strains by in vitro batch cultures. IMPORTANCE Based on our results, we observed a drastic reduction in gut microbiota diversity of children treated with antibiotics, which also affected the abundance of Bifidobacterium, a bacterial genus commonly found in the infant gut. MIC experiments revealed that more than 98% of bifidobacterial strains tested were shown to be inhibited by the AMC antibiotic. Isolation of four insensitive strains and sequencing of their genomes revealed the identity of possible genes involved in AMC resistance mechanisms. Moreover, gut-simulating in vitro experiments revealed that one strain, i.e., Bifidobacterium breve PRL2020, is able to persist in the presence of a complex microbiota combined with AMC antibiotic. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|