Chromosomal polymorphism in the Sporothrix schenckii complex
| Autor: | Fabio Mitsuo Lima, Luciano dos Santos Feitosa, Alexandre Augusto Sasaki, Geisa Ferreira Fernandes, Marjorie Mendes Marini, Anderson Messias Rodrigues, José Franco da Silveira, Zoilo Pires de Camargo, Marcus de Melo Teixeira, Maria Sueli Soares Felipe |
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| Přispěvatelé: | Universidade Federal de São Paulo (UNIFESP), Univ Camilo Castelo Branco, Universidade de Brasília (UnB), Univ Catolica Brasilia |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
lcsh:Medicine
Plant Science Polymerase Chain Reaction Molecular Cell Biology Chromosomal polymorphism Sporothrix schenckii DNA Fungal lcsh:Science Phylogeny Genomic organization Genetics Multidisciplinary Virulence Chromosome Biology Fungal Diseases Electrophoresis Gel Pulsed-Field Blotting Southern Infectious Diseases Medicine Chromosomes Fungal Research Article Genetic Markers Chromosome Structure and Function Mycology Biology Microbiology medicine Internal transcribed spacer Synteny Polymorphism Genetic Sporotrichosis Sporothrix lcsh:R Fungi Botany Genetic Variation biology.organism_classification medicine.disease bacterial infections and mycoses Yeast Genetic marker Karyotyping Fungal Classification Genetic Polymorphism lcsh:Q Population Genetics |
| Zdroj: | PLoS ONE, Vol 9, Iss 1, p e86819 (2014) PLoS ONE Repositório Institucional da UNIFESP Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| ISSN: | 1932-6203 |
| Popis: | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Sporotrichosis is a polymorphic disease caused by a complex of thermodimorphic fungi including S. brasiliensis, S. schenckii sensu stricto (s. str.), S. globosa and S. luriei. Humans and animals can acquire the disease through traumatic inoculation of propagules into the subcutaneous tissue. Despite the importance of sporotrichosis as a disease that can take epidemic proportions there are just a few studies dealing with genetic polymorphisms and genomic architecture of these pathogens. the main objective of this study was to investigate chromosomal polymorphisms and genomic organization among different isolates in the S. schenckii complex. We used pulsed field gel electrophoresis (PFGE) to separate chromosomal fragments of isolated DNA, followed by probe hybridization. Nine loci (beta-tubulin, calmodulin, catalase, chitin synthase 1, Internal Transcribed Spacer, Pho85 cyclin-dependent kinase, protein kinase C Ss-2, G protein a subunit and topoisomerase II) were mapped onto chromosomal bands of Brazilian isolates of S. schenckii s. str. and S. brasiliensis. Our results revealed the presence of intra and interspecies polymorphisms in chromosome number and size. the gene hybridization analysis showed that closely related species in phylogenetic analysis had similar genetic organizations, mostly due to identification of synteny groups in chromosomal bands of similar sizes. Our results bring new insights into the genetic diversity and genome organization among pathogenic species in the Sporothrix schenckii complex. Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Discipline Cellular Biol, São Paulo, Brazil Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Discipline Parasitol, São Paulo, Brazil Univ Camilo Castelo Branco, Ctr Biomed Engn, São Paulo, Brazil Univ Brasilia, Dept Cell Biol, Inst Biol Sci, Brasilia, DF, Brazil Univ Catolica Brasilia, Brasilia, DF, Brazil Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Discipline Cellular Biol, São Paulo, Brazil Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Discipline Parasitol, São Paulo, Brazil Web of Science |
| Databáze: | OpenAIRE |
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