Application of response surface method to evaluate the cytotoxic potency of Ulva fasciata Delile, a marine macro alga
Autor: | Prafulla Kumar Sahu, G. Srinivasa Rao, Mukesh Kumar Das, Lingarkar Silpavathi, K. Mukkanti |
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Rok vydání: | 2014 |
Předmět: |
MTT assay
biology Agricultural and Biological Sciences(all) Cytotoxicity Cell Ulvaceae biology.organism_classification Ulva fasciata Delile Green marine macro alga medicine.anatomical_structure Response surface methodology Cell culture Cancer cell Botany medicine Cytotoxic T cell Original Article Food science General Agricultural and Biological Sciences |
Zdroj: | Saudi Journal of Biological Sciences. 21(6):539-546 |
ISSN: | 1319-562X |
DOI: | 10.1016/j.sjbs.2014.02.003 |
Popis: | Bioprospecting of marine natural products has recently produced a substantial number of drug candidates. Ulva fasciata Delile, belonging to the family Ulvaceae, is a green marine macro alga that grows profusely on the coastal seashore of South India. In the present study, we investigated the in vitro cytotoxic potential of a methanolic extract of U. fasciata Delile (MEUF) using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay against human colon carcinoma (HT-29), human hepatocyte carcinoma (Hep-G2), and human breast carcinoma (MCF-7) cell lines. Response surface methodology (RSM) was applied using central-composite experimental design (CCD) to obtain optimum combined effect of concentration and cancer cells with highest cytotoxicity. The effect of concentration, cancer cell lines as independent variables on absorbance (OD), percent cell survival and percent cell inhibition as dependent variables was investigated. Maximum cytotoxic activity of MEUF was established for Hep-G2 with lowest OD or percent cell survival; highest percent cell inhibition with significant difference (p>0.05) was compared to HT-29 and MCF-7. |
Databáze: | OpenAIRE |
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