Combined Metabolite and Transcriptome Profiling Reveals the Norisoprenoid Responses in Grape Berries to Abscisic Acid and Synthetic Auxin
| Autor: | Chang-Qing Duan, Qi Sun, Yu Wang, Xiang-Yi Li, Zhi-Gang Dong, Nan Meng, Qiu-Hong Pan, Lei He, Xiao-Ping Tang, Simone D. Castellarin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine naphthaleneacetic acid 01 natural sciences Naphthaleneacetic Acids lcsh:Chemistry Transcriptome abscisic acid chemistry.chemical_compound Plant Growth Regulators Gene Expression Regulation Plant Gene expression Vitis MYB lcsh:QH301-705.5 Abscisic acid Spectroscopy chemistry.chemical_classification food and beverages Ripening General Medicine Norisoprenoids Computer Science Applications Horticulture Metabolome grape berry ripening Biology Article Catalysis Inorganic Chemistry 03 medical and health sciences alternative splicing Auxin Metabolomics Physical and Theoretical Chemistry Molecular Biology Gene Indoleacetic Acids Gene Expression Profiling Organic Chemistry fungi norisoprenoid 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 chemistry Fruit gene expression DAP-seq 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 3 International Journal of Molecular Sciences, Vol 22, Iss 1420, p 1420 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22031420 |
| Popis: | The abscisic acid (ABA) increase and auxin decline are both indicators of ripening initiation in grape berry, and norisoprenoid accumulation also starts at around the onset of ripening. However, the relationship between ABA, auxin, and norisoprenoids remains largely unknown, especially at the transcriptome level. To investigate the transcriptional and posttranscriptional regulation of the ABA and synthetic auxin 1-naphthaleneacetic acid (NAA) on norisoprenoid production, we performed time-series GC-MS and RNA-seq analyses on Vitis vinifera L. cv. Cabernet Sauvignon grape berries from pre-veraison to ripening. Higher levels of free norisoprenoids were found in ABA-treated mature berries in two consecutive seasons, and both free and total norisoprenoids were significantly increased by NAA in one season. The expression pattern of known norisoprenoid-associated genes in all samples and the up-regulation of specific alternative splicing isoforms of VviDXS and VviCRTISO in NAA-treated berries were predicted to contribute to the norisoprenoid accumulation in ABA and NAA-treated berries. Combined weighted gene co-expression network analysis (WGCNA) and DNA affinity purification sequencing (DAP-seq) analysis suggested that VviGATA26, and the previously identified switch genes of myb RADIALIS (VIT_207s0005g02730) and MAD-box (VIT_213s0158g00100) could be potential regulators of norisoprenoid accumulation. The positive effects of ABA on free norisoprenoids and NAA on total norisoprenoid accumulation were revealed in the commercially ripening berries. Since the endogenous ABA and auxin are sensitive to environmental factors, this finding provides new insights to develop viticultural practices for managing norisoprenoids in vineyards in response to changing climates. |
| Databáze: | OpenAIRE |
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