In Vitro Cytotoxicity of Trastuzumab (Tz) and Se-Trastuzumab (Se-Tz) Against the Her/2 Breast Cancer Cell Lines JIMT-1 and BT-474
| Autor: | Debalina Goswami Sewell, Julian E. Spallholz, Mallory Boylan, Arun Sharma, Priyanka Bapat |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Receptor
ErbB-2 antibody drug conjugate (ADC) Apoptosis human epidermal growth factor receptor 2 (Her/2) reduced glutathione (GSH) selenium (Se) chemistry.chemical_compound Antineoplastic Agents Immunological Trastuzumab Superoxides Organoselenium Compounds Herceptin® Cytotoxic T cell monoclonal antibody (mab) Biology (General) skin and connective tissue diseases Spectroscopy biology Chemistry Superoxide Caspase 3 Kadcyla® (T-DM-1) General Medicine epidermal growth factor receptor (EGFR) Computer Science Applications Female Antibody Oxidation-Reduction medicine.drug Cell Survival Trastuzumab (Tz) QH301-705.5 Breast Neoplasms Catalysis Article Inorganic Chemistry Selenium superoxide (O2•−) Cell Line Tumor medicine Humans Viability assay Physical and Theoretical Chemistry Molecular Biology QD1-999 Taxane Cell growth Organic Chemistry Tumor progression Drug Resistance Neoplasm biology.protein Cancer research Microscopy Electron Scanning |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 4655, p 4655 (2021) International Journal of Molecular Sciences Volume 22 Issue 9 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Her/2+ breast cancer accounts for ~25% mortality in women and overexpression of Her/2 leads to cell growth and tumor progression. Trastuzumab (Tz) with Taxane is the preferred treatment for Her/2+ patients. However, Tz responsive patients often develop resistance to Tz treatment. Herein, redox selenides (RSe-) were covalently linked to Tz using a selenium (Se)-modified Bolton–Hunter Reagent forming Seleno-Trastuzumab (Se-Tz ~25 µgSe/mg). Se-Tz was compared to Tz and sodium selenite to assess the viability of JIMT-1 and BT-474 cells. Comparative cell viability was examined by microscopy and assessed by fluorometric/enzymatic assays. Se-Tz and selenite redox cycle producing superoxide (O2•−) are more cytotoxic to Tz resistant JIMT-1 and Tz sensitive BT-474 cells than Tz. The results of conjugating redox selenides to Tz suggest a wider application of this technology to other antibodies and targeting molecules. |
| Databáze: | OpenAIRE |
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