Long noncoding RNA ARRDC1-AS1 is activated by STAT1 and exerts oncogenic properties by sponging miR-432-5p/PRMT5 axis in glioma
| Autor: | Zhen-Zhong Zhang, Xin Jin, Qian Zang, Yu-Qiang Lu, Ye Wu, Xiao-Dong Zou |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Transcriptional Activation Protein-Arginine N-Methyltransferases Biophysics Biology medicine.disease_cause Biochemistry 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Transcription (biology) Glioma Cell Line Tumor medicine Humans STAT1 Molecular Biology Gene knockdown Brain Neoplasms Cell Biology medicine.disease Prognosis Long non-coding RNA Antisense RNA Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology STAT1 Transcription Factor 030220 oncology & carcinogenesis Cancer research biology.protein RNA Long Noncoding Carcinogenesis |
| Zdroj: | Biochemical and biophysical research communications. 534 |
| ISSN: | 1090-2104 |
| Popis: | Dysfunction of long noncoding RNA (lncRNA) is associated with tumorigenesis of various malignancies, including glioma. Previously, lncRNA ARRDC1 antisense RNA 1(ARRDC1-AS1) has been reported to be dysregulated in several tumors. However, the roles of ARRDC1-AS1 in glioma have not been investigated. In this study, we firstly reported that ARRDC1-AS1 expression was distinctly increased in both glioma specimens and cell lines, and high ARRDC1-AS1 expression was associated with advanced clinical progression and poor prognosis of glioma patients. Additionally, STAT1 could activate the transcription of ARRDC1-AS1. Functional studies revealed that knockdown of ARRDC1-AS1 suppressed the proliferation, migration and invasion of glioma cells. Mechanisms exploration indicated ARRDC1-AS1 served as a sponge of miR-432-5p to upregulate PRMT5 expressions. Rescue experiments indicated that knockdown of miR-432-5p reversed the inhibiting effects of ARRDC1-AS1 knockdown on glioma cells. Overall, our findings highlighted the importance of STAT1/ARRDC1-AS1/miR-432-5p/PRMT5 axis in glioma progression and offered novel strategies for glioma treatments. |
| Databáze: | OpenAIRE |
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