The formation of occlusion-derived virus is affected by the expression level of ODV-E25
| Autor: | Xiaofeng Wu, Xingwei Xiang, Shaofang Yu, Rui Yang, Xiaolong Hu, Lin Chen |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
Deletion mutant viruses Gene Expression Spodoptera Biology Virus Replication Virus Viral Proteins Virology Sf9 Cells Polyhedrin Animals Promoter Regions Genetic Cell Nucleus Recombination Genetic Virus Assembly Genetic Complementation Test fungi Virion Transfection Viral DNA replication Molecular biology Microvesicles Microscopy Electron Infectious Diseases Baculoviridae Gene Deletion Core gene |
| Zdroj: | Virus Research. 173:404-414 |
| ISSN: | 0168-1702 |
| DOI: | 10.1016/j.virusres.2012.12.016 |
| Popis: | Odv-e25 is a core gene of baculoviruses and encodes a 25.5 kDa protein located on both budded virus (BV) and occlusion-derived virus (ODV). Our previous study demonstrated that ODV-E25 was required for the formation of intranuclear microvesicles and ODV, and an odv-e25 deletion mutant could be rescued by re-expression of odv-e25 under its native promoter. To investigate the functions of ODV-E25 expression level on ODV formation, the promoter of ie-1 (pIE1), the odv-e25 native promoter, and the polyhedrin promoter (pPH) were used to direct odv-e25 expression. Our results showed that the production of ODV-E25 under its native promoter was higher than that under pIE1 but lower than that under pPH. Viral DNA replication and budded viruses (BVs) production showed that expression of odv-e25 under pIE1 and pPH could not completely repair the defects caused by the deletion of ODV-E25, while expression under its native promoter did. Electron microscopy showed that intranuclear microvesicles were found in all the constructs transfected cells except the odv-e25-null virus. However, mature ODVs only were detected in cells transfected with virus in which odv-e25 was expressed under its native or polyhedrin promoter. These results indicated that the formation occlusion-derived virus was affected by the expression level of ODV-E25. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect