Receptor-Mediated Host Cell Preference of a Bat-Derived Filovirus, Lloviu Virus
| Autor: | Takeshi Saito, Yoshihiro Takadate, Rashid Manzoor, Hiroko Miyamoto, Manabu Igarashi, Ayato Takada, Yurie Kida, Hirohito Ogawa, Junki Maruyama, Masahiro Kajihara, Tatsunari Kondoh |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) glycoprotein filovirus congenital hereditary and neonatal diseases and abnormalities Miniopterus sp viruses 030106 microbiology host range bat Biology medicine.disease_cause Microbiology Article Virus Marburg virus 03 medical and health sciences Virology hemic and lymphatic diseases medicine insectivorous bat lcsh:QH301-705.5 Tropism Lloviu virus Ebola virus Niemann–Pick C1 nutritional and metabolic diseases biology.organism_classification 030104 developmental biology lcsh:Biology (General) Vesicular stomatitis virus Vero cell NPC1 lloviu virus |
| Zdroj: | Microorganisms, Vol 8, Iss 1530, p 1530 (2020) Microorganisms Volume 8 Issue 10 |
| ISSN: | 2076-2607 |
| Popis: | Lloviu virus (LLOV), a bat-derived filovirus that is phylogenetically distinct from human pathogenic filoviruses such as Ebola virus (EBOV) and Marburg virus (MARV), was discovered in Europe. However, since infectious LLOV has never been isolated, the biological properties of this virus remain poorly understood. We found that vesicular stomatitis virus (VSV) pseudotyped with the glycoprotein (GP) of LLOV (VSV&ndash LLOV) showed higher infectivity in one bat (Miniopterus sp.)-derived cell line than in the other bat-derived cell lines tested, which was distinct from the tropism of VSV pseudotyped with EBOV (VSV&ndash EBOV) and MARV GPs. We then focused on the interaction between GP and Niemann&ndash Pick C1 (NPC1) protein, one of the cellular receptors of filoviruses. We introduced the Miniopterus bat and human NPC1 genes into NPC1-knockout Vero E6 cells and their susceptibilities to the viruses were compared. The cell line expressing the bat NPC1 showed higher susceptibility to VSV&ndash LLOV than that expressing human NPC1, whereas the opposite preference was seen for VSV&ndash EBOV. Using a site-directed mutagenesis approach, amino acid residues involved in the differential tropism were identified in the NPC1 and GP molecules. Our results suggest that the interaction between GP and NPC1 is an important factor in the tropism of LLOV to a particular bat species. |
| Databáze: | OpenAIRE |
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