Decreased glutathione transferase levels in rd1/rd1 mouse retina: Replenishment protects photoreceptors in retinal explants
| Autor: | Satpal Ahuja, P Ahuja, Per Ekström, A.R. Caffe, T. van Veen |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Retinal degeneration
Gene isoform Blotting Western Retina Mice Organ Culture Techniques Western blot medicine Animals Ganglion cell layer Glutathione Transferase Mice Inbred C3H biology medicine.diagnostic_test General Neuroscience Retinal Degeneration medicine.disease Immunohistochemistry Molecular biology eye diseases Isoenzymes Blot Oxidative Stress medicine.anatomical_structure Glutathione S-transferase Apoptosis biology.protein sense organs Photoreceptor Cells Vertebrate |
| Zdroj: | Neuroscience. 131:935-943 |
| ISSN: | 0306-4522 |
| DOI: | 10.1016/j.neuroscience.2004.11.012 |
| Popis: | Currently much attention is focused on glutathione S transferase (GST)-induced suppression of apoptosis. The objective of our studies was therefore to see if GST isoenzymes rescue photoreceptors in retinal explants from rd1/rd1 mice, in which photoreceptors degenerate rapidly. Eyes from C3H rd1/rd1 and +/+ mice were collected at various time points between postnatal day (PN) 2 and PN28. Localization and content of alpha-GST and mu-GST was investigated by immunofluorescence and semi-quantitative Western blot analysis, respectively. In addition, PN2 and PN7 retinal explants were cultured till PN28, during which they were treated with 10 ng/ml alpha-GST or mu-GST. The spatiotemporal expression of both GST isoforms was closely similar: early presence in ganglion cell layer after which staining became restricted to Muller cells (particularly in the endfeet) and horizontal cell fibers in both rd1/rd1 and +/+. Doublets of alpha-GST and mu-GST were detected by Western blot analysis. Densitometry of these bands indicated steady reduction of alpha-GST content in rd1/rd1 retina starting from the second postnatal week. When alpha-GST and mu-GST were added exogenously to rd1/rd1 explants, photoreceptor rescue was produced that was more prominent in PN2 than in PN7 explants and more effective by alpha-GST than mu-GST. We propose that alpha-GST neuroprotection is mediated by reduction of tissue oxidative stress. |
| Databáze: | OpenAIRE |
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