Metabolomics Reveals the Alteration of Metabolic Pathway by Alpha-Melanocyte-Stimulating Hormone in B16F10 Melanoma Cells

Autor:	Seong-Eun Park, Kyung Mok Park, Seung-Ho Seo, Seo Yeon Shin, Jae Kwon Jo, Eun-Ju Kim, Hong-Seok Son
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	Fumaric acid melanoma cell Melanoma Experimental Pharmaceutical Science amino acid metabolism 01 natural sciences Article Analytical Chemistry Melanin lcsh:QD241-441 α-melanocyte-stimulating hormone 03 medical and health sciences chemistry.chemical_compound Mice lcsh:Organic chemistry Oxaloacetic acid Cell Line Tumor Drug Discovery energy metabolism Animals Humans Physical and Theoretical Chemistry skin and connective tissue diseases 030304 developmental biology chemistry.chemical_classification Melanins 0303 health sciences integumentary system Pigmentation 010401 analytical chemistry Organic Chemistry metabolomics alpha-Melanocyte-stimulating hormone 0104 chemical sciences Amino acid Citric acid cycle Metabolic pathway chemistry Biochemistry Chemistry (miscellaneous) alpha-MSH Molecular Medicine Malic acid sense organs hormones hormone substitutes and hormone antagonists Metabolic Networks and Pathways Signal Transduction
Zdroj:	Molecules Molecules, Vol 25, Iss 3384, p 3384 (2020) Volume 25 Issue 15
ISSN:	1420-3049
Popis:	The purpose of this study was to understand the changes of metabolic pathway induced by alpha-melanocyte-stimulating hormone (&alpha MSH) in B16F10 melanoma cells in an untargeted metabolomics approach. Cells were treated with 100 nM of &alpha MSH and then incubated for 48 h. &alpha MSH increased tyrosinase activity and melanin content by 56.5 and 61.7%, respectively, compared to untreated cells after 48 h of cultivation. The clear separation between groups was observed in the principal component analysis score plot, indicating that the levels of metabolites of melanoma cells were altered by treatment with &alpha MSH. Metabolic pathways affected by &alpha MSH were involved in some amino acid metabolisms. The increased levels of fumaric acid, malic acid, oxaloacetic acid and citric acid related to the citric acid cycle pathway after &alpha MSH treatment suggested enhanced energy metabolism. Metabolic pathways altered by &alpha MSH treatment can provide useful information to develop new skin pigmentation inhibitors or anti-obesity drugs.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45b99bc53a13018c4e811c6ce6378314 http://europepmc.org/articles/PMC7436294 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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